FN Archimer Export Format
PT J
TI DNA hybridization mechanism in an interfacial environment: What hides beneath first order k (s(-1)) kinetic constant?
BT 
AF LAZERGES, M.
   PERROT, H.
   RABEHAGASOA, N.
   COMPERE, Chantal
   DREANNO, Catherine
   MUCIO PEDROSO, M.
   FARIA, R. C.
   BUENO, P. R.
AS 1:1,2;2:1,2;3:3;4:3;5:3;6:4;7:4;8:5;
FF 1:;2:;3:;4:PDG-REM-RDT-IC;5:PDG-REM-RDT-IC;6:;7:;8:;
C1 CNRS, UPR 15, Lab Interfaces & Syst Electrochim, F-75252 Paris, France.
   Univ Paris 06, LISE, F-75252 Paris, France.
   IFREMER, Ctr Brest, Serv Interfaces & Capteurs, ZI Pointe Diable, F-29280 Plouzane, France.
   Univ Fed Sao Carlos, Dept Quim, BR-13560905 Sao Paulo, Brazil.
   Univ Estadual Paulista, Dept Fisicoquim, Inst Quim, BR-14800900 Sao Paulo, Brazil.
C2 CNRS, FRANCE
   UNIV PARIS 06, FRANCE
   IFREMER, FRANCE
   UNIV FED SAO CARLOS, BRAZIL
   UNIV ESTADUAL PAULISTA, BRAZIL
SI BREST
SE PDG-REM-RDT-IC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 3.54
TC 5
UR https://archimer.ifremer.fr/doc/00101/21195/19415.pdf
LA English
DT Article
DE ;Biosensors;DNA;Hybridization kinetics;Quartz crystal microbalance
AB The scientific question addressed in this work is: what hides beneath first order kinetic constant k (s(-1)) measured for hybridization of a DNA target on a biosensor surface. Kinetics hybridization curves were established with a 27 MHz quartz microbalance (9 MHz, third harmonic) biosensor, constituted of a 20-base probe monolayer deposited on a gold covered quartz surface. Kinetics analysis, by a known two-step adsorption-hybridization mechanism, is well appropriate to fit properly hybridization kinetics curves, for complementary 20-base to 40-base targets over two concentration decades. It was found that the K-1 (M-1) adsorption constant, relevant to the first step, concerns an equilibrium between non hybridized targets and hybridized pre-complex and increases with DNA target length. It was established that k(2) (s(-1)), relevant to irreversible formation of a stable duplex, varies in an opposite way to K-1 with DNA target length. (C) 2012 Published by Elsevier B.V.
PY 2012
PD AUG
SO Sensors And Actuators B-chemical
SN 0925-4005
PU Elsevier Science Sa
VL 171
UT 000308572700066
BP 522
EP 527
DI 10.1016/j.snb.2012.05.023
ID 21195
ER

EF