FN Archimer Export Format
PT J
TI Thiol- and Biotin-Labeled Probes for Oligonucleotide Quartz Crystal Microbalance Biosensors of Microalga Alexandrium Minutum
BT 
AF LAZERGES, Mathieu
   PERROT, Hubert
   RABEHAGASOA, Niriniony
   COMPERE, Chantal
AS 1:1,2;2:1,2;3:1,2;4:3;
FF 1:;2:;3:;4:PDG-REM-RDT-IC;
C1 Centre National de la Recherche Scientifique (CNRS), Unité Propre de Recherche (UPR) 15, Laboratoire Interfaces et Systèmes Electrochimiques, 4 place Jussieu, Paris 75252, France
   Université Pierre et Marie Curie, LISE, 4 place Jussieu, Paris 75252, France
   Institut Français de Recherche pour l’Exploitation de la Mer (IFREMER), Centre de Brest Service Interfaces et Capteurs, Z.I. pointe du diable, Plouzané 29280, France
C2 CNRS, FRANCE
   UNIV PARIS 06, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-REM-RDT-IC
TC 0
UR https://archimer.ifremer.fr/doc/00107/21810/19385.pdf
LA English
DT Article
DE ;Alexandrium Minutum;avidin;biosensor;biotin;DNA;quartz crystal miocrobalance;self-assembled monolayer;thiol
AB Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the complementary 20-base target, have been monitored in situ with a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments. DNA recognition by the two biosensors is efficient and selective. Hybridization kinetic curves indicate that the biosensor designed with the thiol-labeled probe is more sensitive, and that the biosensor designed with the biotin-labeled probe has a shorter time response and a higher hybridization efficiency.
PY 2012
PD JUN
SO Biosensors
SN 2079-6374
PU MDPI
VL 2
IS 3
BP 245
EP 254
DI 10.3390/bios2030245
ID 21810
ER

EF