FN Archimer Export Format
PT J
TI Improvement of chilled seabass sperm conservation using a cell culture medium
BT 
AF FAUVEL, Christian
   BORYSHPOLETS, Sergey
   COSSON, J.
   LEEDY, J. G. Wilson
   LABBE, C.
   HAFFRAY, Pierrick
   SUQUET, Marc
AS 1:1;2:2;3:2;4:3;5:4;6:5;7:6;
FF 1:PDG-RBE-BOME-LALR;2:;3:;4:;5:;6:;7:PDG-RBE-PFOM-PI;
C1 IFREMER, CNRS, IRD, Stn Expt Aquaculture,UM1,UM2,UMR ECOSYM 5119, F-34250 Palavas Les Flots, France.
   Univ S Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, S Bohemian Res Ctr Aquaculture & Biodivers Hydroc, Vodnany, Czech Republic.
   Univ Idaho, Dept Biol Sci, Ctr Reprod Biol, Moscow, ID 83843 USA.
   INRA, UR Scribe 1037, F-35042 Rennes, France.
   SYSAAF, Scribe, Rennes, France.
   IFREMER, Stn Expt Argenton, UMR 100, Argenton, France.
C2 IFREMER, FRANCE
   UNIV S BOHEMIA, CZECH REPUBLIC
   UNIV IDAHO, USA
   INRA, FRANCE
   SYSAAF, FRANCE
   IFREMER, FRANCE
SI PALAVAS
   ARGENTON
SE PDG-RBE-BOME-LALR
   PDG-RBE-PFOM-PI
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-p187
   copubli-europe
   copubli-int-hors-europe
IF 0.9
TC 13
UR https://archimer.ifremer.fr/doc/00111/22221/20066.pdf
LA English
DT Article
AB The sperm of seabass is very fragile and it quickly loses its ability to fertilize after collection either if kept undiluted or in classic saline media. In order to avoid cryopreservation when only short conservation is required, the process of sperm management including sperm collection, sperm dilution rate in storage medium and storage medium composition, was subject to experimental trials. A concentration of 20% urine generated a low pH of seminal fluid, and it immediately altered the motility ability. However, pH did not seem to be the key agent of motility prevention since sperm dilution in Leibovitz culture medium (L15) or in classic saline medium both presenting a similar low pH (7.3) did not affect motility. L15 increased the duration of sperm survival by 2 days at 4 degrees C after collection. Moreover, dilution could be restricted to 1 : 3 (v : v) for conservation of chilled sperm. Chilled sperm could be cryopreserved with no more damages than those observed after freezing of fresh sperm.
PY 2012
PD DEC
SO Journal Of Applied Ichthyology
SN 0175-8659
PU Wiley-blackwell
VL 28
IS 6
UT 000310976600020
BP 961
EP 966
DI 10.1111/jai.12071
ID 22221
ER

EF