FN Archimer Export Format PT J TI The Protist Ribosomal Reference database (PR2): a catalog of unicellular eukaryote Small Sub-Unit rRNA sequences with curated taxonomy BT AF GUILLOU, Laure BACHAR, Dipankar AUDIC, S BASS, David BERNEY, Cedric BITTNER, Lucie BOUTTE, Christophe BURGAUD, Gaetan DE VARGAS, Colomban DECELLE, Johan DEL CAMPO, Javier DOLAN, John R. DUNTHORN, Micah EDVARDSEN, Bente HOLZMANN, Maria KOOISTRA, Wiebe H. C. F. LARA, Enrique LE BESCOT, Noan LOGARES, Ramiro MAHE, F MASSANA, Ramon MONTRESOR, Marina MORARD, Raphael NOT, Fabrice PAWLOWSKI, Jan PROBERT, Ian SAUVADET, Anne-Laure SIANO, Raffaele STOECK, Thorsten VAULOT, Daniel ZIMMERMANN, Pascal CHRISTEN, Richard AS 1:1,2;2:3,4;3:1,2;4:5;5:5;6:1,2;7:1,2;8:6;9:1,2;10:1,2;11:7;12:8,9;13:10;14:11;15:12;16:13;17:14;18:1,2;19:7;20:1,2;21:7;22:13;23:1,2;24:1,2;25:12;26:15;27:1,2;28:16;29:10;30:1,2;31:17;32:3,4; FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;12:;13:;14:;15:;16:;17:;18:;19:;20:;21:;22:;23:;24:;25:;26:;27:;28:PDG-ODE-DYNECO-PELAGOS;29:;30:;31:;32:; C1 CNRS, UMR Adaptat & Divers Milieu Marin 7144, F-29682 Roscoff, France. Univ Paris 06, UMR 7144, Stn Biol Roscoff, F-29682 Roscoff, France. CNRS, UMR 7138, F-06108 Nice 02, France. Univ Nice Sophia Antipolis, UMR 7138, F-06108 Nice 02, France. Nat Hist Museum, Dept Life Sci, London SW7 5BD, England. ESMISAB, Lab Univ Biodivers & Ecol Microbienne EA3882, F-29280 Plouzane, France. CSIC, Inst Ciencies Mar, Dept Marine Biol & Oceanog, Barcelona, Catalonia, Spain. Univ Paris 06, Lab Oceanog Villefranche, F-06230 Villefranche Sur Mer, France. CNRS, UMR7093, Stn Zool, F-06230 Villefranche Sur Mer, France. Univ Kaiserslautern, Dept Ecol, D-67663 Kaiserslautern, Germany. Univ Oslo, Dept Biol, NO-0316 Oslo, Norway. Univ Geneva, Dept Genet & Evolut, CH-1211 Geneva 4, Switzerland. Stn Zool Anton Dohrn, I-80121 Naples, Italy. Univ Neuchatel, Lab Soil Biol, CH-2000 Neuchatel, Switzerland. CNRS, FR2424, Stn Biol Roscoff, F-29682 Roscoff, France. IFREMER, Ctr Brest, F-29280 Plouzane, France. Point Competence Informat, F-56850 Caudan, France. C2 CNRS, FRANCE UNIV PARIS 06, FRANCE CNRS, FRANCE UNIV NICE, FRANCE NHM, UK ESMISAB, FRANCE CSIC, SPAIN UNIV PARIS 06, FRANCE CNRS, FRANCE UNIV KAISERSLAUTERN, GERMANY UNIV OSLO, NORWAY UNIV GENEVA, SWITZERLAND STN ZOOL ANTON DOHRN, ITALY UNIV NEUCHATEL, SWITZERLAND CNRS, FRANCE IFREMER, FRANCE POINT COMPETENCE INFORMAT, FRANCE SI BREST SE PDG-ODE-DYNECO-PELAGOS CNRS IN WOS Ifremer jusqu'en 2018 copubli-france copubli-europe copubli-univ-france copubli-int-hors-europe IF 8.808 TC 646 UR https://archimer.ifremer.fr/doc/00114/22492/20174.pdf LA English DT Article AB The interrogation of genetic markers in environmental meta-barcoding studies is currently seriously hindered by the lack of taxonomically curated reference data sets for the targeted genes. The Protist Ribosomal Reference database (PR2, http://ssurrna. org/) provides a unique access to eukaryotic small sub-unit (SSU) ribosomal RNA and DNA sequences, with curated taxonomy. The database mainly consists of nuclear-encoded protistan sequences. However, metazoans, land plants, macrosporic fungi and eukaryotic organelles (mitochondrion, plastid and others) are also included because they are useful for the analysis of hightroughput sequencing data sets. Introns and putative chimeric sequences have been also carefully checked. Taxonomic assignation of sequences consists of eight unique taxonomic fields. In total, 136 866 sequences are nuclear encoded, 45 708 (36 501 mitochondrial and 9657 chloroplastic) are from organelles, the remaining being putative chimeric sequences. The website allows the users to download sequences from the entire and partial databases (including representative sequences after clustering at a given level of similarity). Different web tools also allow searches by sequence similarity. The presence of both rRNA and rDNA sequences, taking into account introns (crucial for eukaryotic sequences), a normalized eight terms ranked-taxonomy and updates of new GenBank releases were made possible by a long-term collaboration between experts in taxonomy and computer scientists. PY 2013 PD JAN SO Nucleic Acids Research SN 0305-1048 PU Oxford Univ Press VL 41 IS D1 UT 000312893300085 DI 10.1093/nar/gks1160 ID 22492 ER EF