FN Archimer Export Format PT J TI Study of the antioxidant capacity in gills of the Pacific oyster Crassostrea gigas in link with its reproductive investment BT AF BEGUEL, Jean-Philippe HUVET, Arnaud QUILLIEN, Virgile LAMBERT, Christophe FABIOUX, Caroline AS 1:1;2:2;3:2;4:1;5:1; FF 1:;2:PDG-RBE-PFOM-PI;3:PDG-RBE-PFOM-PI;4:;5:; C1 Univ Bretagne Occidentale, Inst Univ Europeen Mer, Lab Sci Environm Marin, CNRS UBO IRD IFREMER,UMR 6539, F-29280 Plouzane, France. CNRS UBO IRD IFREMER, UMR 6539, Lab Physiol Invertebres, IFREMER, F-29280 Plouzane, France. C2 UBO, FRANCE IFREMER, FRANCE SI BREST SE PDG-RBE-PFOM-PI IUEM IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 2.829 TC 21 UR https://archimer.ifremer.fr/doc/00116/22744/21494.pdf LA English DT Article DE ;Antioxidant capacity;Crassostrea gigas;Reproduction;RNA interference;ROS AB Energy allocation principle is a core element of life-history theory in which "the cost of reproduction" corresponds to an acceleration of senescence caused by an increase in reproductive investment. In the "theory of aging", senescence is mainly due to the degradation of lipids, proteins and DNA by reactive oxygen species (ROS), by-products of oxidative metabolism. Some studies have shown that oxidative stress susceptibility could be a cost of reproduction. The present study investigates the effect of reproductive investment on antioxidant capacity in the gills of a species with a very high reproductive investment, the Pacific oyster Crassostrea gigas. We used RNA interference targeting the oyster vasa-like gene (Oyvlg) to produce oysters with contrasted reproductive investment. Antioxidant capacity was studied by measuring the mRNA levels of genes encoding major antioxidant enzymes, and the activity of these enzymes. The highest reproductive investment was associated with the highest transcript levels for glutathione peroxidase and extra-cellular and mitochondrial superoxide dismutase. In contrast, lipid peroxidation did not show any sign of oxidative damage whatever the reproductive investment. Up-regulation of certain genes encoding enzymes involved in the first step of ROS detoxification could therefore be a part of the organism's strategy for managing the pro-oxidant species produced by heavy reproductive investment. (C) 2012 Elsevier Inc. All rights reserved. PY 2013 PD JAN SO Comparative Biochemistry And Physiology C-toxicology & Pharmacology SN 1532-0456 PU Elsevier Science Inc VL 157 IS 1 UT 000312470600008 BP 63 EP 71 DI 10.1016/j.cbpc.2012.10.004 ID 22744 ER EF