FN Archimer Export Format
PT J
TI Characterization of the protozoan parasite Marteilia refringens infecting the dwarf oyster Ostrea stentina in Tunisia
BT 
AF ELGHARSALLI, Refka
   ALOUI-BEJAOUI, Nejla
   SALAH, Hedi
   CHOLLET, Bruno
   JOLY, Jean-Pierre
   ROBERT, Maeva
   COURALEAU, Yann
   ARZUL, Isabelle
AS 1:1;2:1;3:1;4:2;5:2;6:2;7:2;8:2;
FF 1:;2:;3:;4:PDG-RBE-SG2M-LGPMM;5:PDG-RBE-SG2M-LGPMM;6:PDG-RBE-SG2M-LGPMM;7:PDG-RBE-AGSAE-LGP;8:PDG-RBE-SG2M-LGPMM;
C1 Inst Natl Agron Tunisie, Tunis 1082, Tunisia.
   IFREMER, Lab Genet & Pathol, F-17390 La, Tremblade, France.
C2 INAT, TUNISIA
   IFREMER, FRANCE
SI LA TREMBLADE
SE PDG-RBE-SG2M-LGPMM
   PDG-RBE-AGSAE-LGP
IN WOS Ifremer jusqu'en 2018
   copubli-int-hors-europe
   copubli-sud
IF 2.601
TC 11
UR https://archimer.ifremer.fr/doc/00124/23538/21422.pdf
LA English
DT Article
DE ;Marteilia refringens;Molecular diagnosis;IGS;ITS;Transmission electron microscopy;Ostrea stentina
AB Marteilia refringens is a protozoan parasite recognized as a significant pathogen of the European flat oyster Ostrea edulis. The life cycle of this species is still poorly known, although there is evidence of the need for intermediate host(s). In the present study, we have used molecular approaches to identify this parasite in samples of the dwarf oyster Ostrea stentina after reports of massive mortality along the Tunisian coasts. In 2009 we evaluated the status of O. stentina from Monastir and checked if there was an infection with M. refringens, using polymerase chain reaction assays. Of the 103 tested O. stentina, 85 were PCRpositive using a Marteilia genus-specific assay. Additional assays were subsequently carried out on some samples collected in 2010 in Monastir and processed for histology, transmission electron microscopy and complementary molecular analyses. PCR was carried out to amplify the IGS and ITS regions. Histological and transmission electron microscopy analyses allowed us to confirm the presence of this parasite in the digestive gland tissue of O. stentina and to characterize it at the ultrastructural level. This is the first record of the occurrence of M. refringens in the oyster O. stentina along the Tunisian coasts.
PY 2013
PD FEB
SO Journal Of Invertebrate Pathology
SN 0022-2011
PU Academic Press Inc Elsevier Science
VL 112
IS 2
UT 000314381000013
BP 175
EP 183
DI 10.1016/j.jip.2012.11.004
ID 23538
ER

EF