FN Archimer Export Format
PT J
TI First molecular evidence of cross-species induction of metalloprotease gene expression in Vibrio strains pathogenic for Pacific oyster Crassostrea gigas involving a quorum sensing system
BT 
AF DE DECKER, Sophie
   REYNAUD, Yann
   SAULNIER, Denis
   REYNAUD, Yann
AS 1:;2:;3:;4:;
FF 1:;2:PDG-RBE-SG2M-LGPMM;3:PDG-RBE-RMPF-EP;4:PDG-RBE-MASAE-LSEM;
C1 IFREMER, Lab Genet & Pathol, F-17390 La Tremblade, France.
   Ctr Ifremer Tahiti, F-98719 Tahiti, Polynesie, France.
C2 IFREMER, FRANCE
   IFREMER, FRANCE
SI TAHITI
   LA TREMBLADE
   NANTES
SE PDG-RBE-SG2M-LGPMM
   PDG-RBE-RMPF-EP
   PDG-RBE-MASAE-LSEM
IN WOS Ifremer jusqu'en 2018
IF 1.828
TC 18
UR https://archimer.ifremer.fr/doc/00124/23539/21381.pdf
LA English
DT Article
DE ;Quorum sensing;Crassostrea gigas;Vibrio;Metalloprotease;Gene expression
AB The aim of that studywas to explore the hypothesis that a quorumsensingmechanismmodulatesmetalloprotease gene expression of two Vibrio species pathogenic for oyster, at intraspecific (Vibrio splendidus) and interspecific (V. splendidus affecting V. aestuarianus) levels. Metalloprotease activities (Vsm for V. splendidus LGP32 and Vam for V. aestuarianus 02/041) and growth curves obtained by real-time PCR assay revealed cell density-dependent metalloprotease inductions triggered between 12 h and 16 h of culture. A quorum sensing assay was then developed using a conditioned medium prepared from supernatant of a mutant strain of V. splendidus LGP32 unable to produce metalloprotease. Specific real-time qPCR assays targeting metalloprotease genes vsm and vam were performed. A relative increase in expressionwas observed for vsm and, particularly, vam in the presence of the conditioned medium, probably controlled by a quorumsensing system. The study revealed intraspecific and interspecific modulation of expression in metalloprotease genes vsm and vam in V. splendidus and V. aestuarianus. Our experiments showed for the first time that V. splendidus is able to produce an autoinducer-like substance that displays intra- and interspecific effects on the expression of two different metalloprotease genes: vsm and vam
PY 2013
PD MAY
SO Aquaculture
SN 0044-8486
PU Elsevier Science Bv
VL 392
UT 000317567200001
BP 1
EP 7
DI 10.1016/j.aquaculture.2013.01.033
ID 23539
ER

EF