FN Archimer Export Format PT J TI Comparative analysis of male germ cell proliferation and apoptosis in wild and captive Atlantic bluefin tuna (Thunnus thynnus L.) BT AF ZUPA, R. FAUVEL, Christian MYLONAS, C. C. SANTAMARIA, N. VALENTINI, L. POUSIS, C. PAPADAKI, M. SUQUET, Marc DE LA GANDARA, F. BELLO, G. METRIO, G. CORRIERO, A. AS 1:1;2:2;3:3;4:1;5:1;6:1;7:3;8:2;9:4;10:1;11:1;12:1; FF 1:;2:PDG-RBE-BOME-LALR;3:;4:;5:;6:;7:;8:PDG-RBE-PFOM-PI;9:;10:;11:;12:; C1 Univ Bari Aldo Moro, Dept Anim Prod, I-70010 Valenzano, BA, Italy. UM2 UM1 CNRS IRD IFREMER, Stn Ifremer, UMR ECOSYM 5119, Palavas Les Flots, France. Hellen Ctr Marine Res, Inst Aquaculture, Iraklion, Crete, Greece. Ctr Oceanog Murcia, Inst Espanol Oceanog, Puerto De Mazarron, Murcia, Spain. C2 UNIV BARI, ITALY IFREMER, FRANCE HELLEN CTR MARINE RES, GREECE IEO, SPAIN SI PALAVAS ARGENTON SE PDG-RBE-BOME-LALR PDG-RBE-PFOM-PI IN WOS Ifremer jusqu'en 2018 copubli-europe IF 0.903 TC 18 UR https://archimer.ifremer.fr/doc/00125/23579/25462.pdf LA English DT Article AB The most commonly observed reproductive dysfunction in male fishes reared in captivity is reduction in sperm volume and quality. The Atlantic bluefin tuna Thunnus thynnus (Osteichthyes: Scombridae) is one of the few large pelagic and migratory marine fishes maintained in captivity with the purpose of establishing breeding populations to support an aquaculture industry. The objectives of the present study were to compare male germ cell proliferation and apoptosis between wild and captive individuals at two different phases of the spermatogenetic cycle, and to evaluate sperm motility characteristics of captive individuals. Histological observations were performed to analyze testicular activity, and germ cell proliferation and apoptosis were evaluated through the immunohistochemical detection of proliferating cell nuclear antigen (PCNA) and the terminal deoxynucleotidyl transferasemediated d'UTP nick end labeling (TUNEL) method, respectively. Computer-assisted sperm analysis (CASA) was used to evaluate sperm motility. Results showed that germ cell proliferation was delayed and germ cell apoptosis increased in captive animals relative to wild individuals. Sperm motility of samples obtained from captive individuals was anomalous, both in terms of motility duration and swimming efficiency. Thus it appears that rearing in captivity impairs male reproductive function through, at least, changes in germ cell proliferation and apoptosis. PY 2013 PD FEB SO Journal Of Applied Ichthyology SN 0175-8659 PU Wiley-blackwell VL 29 IS 1 UT 000313553400012 BP 71 EP 81 DI 10.1111/j.1439-0426.2012.02045.x ID 23579 ER EF