FN Archimer Export Format
PT J
TI Cryopreservation of gametophytes of Laminaria digitata (L) lamouroux by encapsulation dehydration
BT 
AF VIGNERON, Thierry
   ARBAULT, Suzanne
   KAAS, Raymond
AS 1:;2:1;3:1;
FF 1:;2:;3:;
C1 Ifremer, France
C2 IFREMER, FRANCE
SI NANTES
IF 1.053
TC 20
UR https://archimer.ifremer.fr/doc/00125/23643/21691.pdf
LA English
DT Article
DE ;alga;Laminaria digitata;gametophyte;cryopreservation;encapsulation;dehydration.
AB A cryopreservation procedure was developed for gametophytes of Laminaria digitata using an encapsulation-dehydration technique. Cells were encapsulated in calcium alginate beads pretreated for 6 h in liquid medium containing 0.3 to 0.5 M sucrose and then dehydrated. Freezing was performed in two steps, the first consisting of a slow temperature decrease from 19°C to -40°C and the second of direct immersion of samples in liquid nitrogen. Thawing was performed in a water bath at 40°C for 2 min. Survival rates were between 25 and 75% depending on age, sex and stress. The best results were obtained with alginate beads pretreated for 6 h in a 0.3-0.4 M sucrose solution and dehydrated down to 40% of the initial wet weight. Recovery time was about two weeks.
PY 1997
PD MAR
SO Cryo-letters
SN 0143-2044
VL 18
IS 2
UT A1997WR27500004
BP 93
EP 98
ID 23643
ER

EF