FN Archimer Export Format
PT J
TI Microsatellite markers developed through pyrosequencing allow clonal discrimination in the invasive alga Caulerpa taxifolia
BT 
AF ARNAUD-HAOND, Sophie
   CANDEIAS, Rui
   SERRAO, Ester A.
   TEIXEIRA, Sara
AS 1:1;2:2;3:2;4:2;
FF 1:PDG-RBE-HM-RHSETE;2:;3:;4:;
C1 IFREMER, Dept RBE, UMR Ecosyst Marin Exploite EME 212, F-34203 Sete, France.
   Univ Algarve, Fac Ciencias & Tecnol, CCMAR, CIMAR Lab Associado, P-8005139 Faro, Portugal.
C2 IFREMER, FRANCE
   UNIV ALGARVE, PORTUGAL
SI SETE
SE PDG-RBE-HM-RHSETE
IN WOS Ifremer jusqu'en 2018
   copubli-europe
IF 1.136
TC 5
UR https://archimer.ifremer.fr/doc/00126/23720/21598.pdf
LA English
DT Article
DE ;Caulerpa taxifolia;Algae;Invasion;Mediterranean;Queensland;New South Wales;Seagrass
AB Polymorphic microsatellites were developed for the invasive green algae Caulerpa taxifolia using next generation DNA sequencing. Results showed a limited rate of microsatellites for the amount of sequences, possibly explaining failure of previous attempts for microsatellite development through classical methods. Eight polymorphic loci were selected that exhibited polymorphism and a null or negligible rate of amplification failure. The number of alleles per locus ranged from two to seven. The reconstruction of Multi Locus Genotypes and the heterozygosity and departure from Hardy-Weinberg equilibrium confirmed the influence of clonal reproduction and showed the usefulness of this set of markers to successfully discriminate clonal lineages and analyze the clonal and genetic composition of algal beds. These markers will be used to further investigate the clonal composition and genetic structure in populations of Caulerpa taxifolia and to attempt retracing the origin of and pathways followed by invasive clonal lineages.
PY 2013
PD SEP
SO Conservation Genetics Resources
SN 1877-7252
PU Springer
VL 5
IS 3
UT 000322619900017
BP 667
EP 669
DI 10.1007/s12686-013-9878-8
ID 23720
ER

EF