FN Archimer Export Format
PT J
TI Metagenomes of the Picoalga Bathycoccus from the Chile Coastal Upwelling
BT 
AF VAULOT, Daniel
   LEPERE, Cecile
   TOULZA, Eve
   DE LA IGLESIA, Rodrigo
   POULAIN, Julie
   GABOYER, Frederic
   MOREAU, Herve
   VANDEPOELE, Klaas
   ULLOA, Osvaldo
   GAVORY, Frederick
   PIGANEAU, Gwenael
AS 1:1,2;2:1,2;3:3,4;4:5,6;5:7;6:1,2;7:3,4;8:8,9;9:5,6;10:7;11:3,4;
FF 1:;2:;3:;4:;5:;6:;7:;8:;9:;10:;11:;
C1 Univ Paris 06, UPMC, Roscoff, France.
   CNRS, Biol Stn, UMR 7144, Roscoff, France.
   CNRS, Banyuls Sur Mer, France.
   Univ Paris 06, UPMC, OOB, UMR 7232, Banyuls Sur Mer, France.
   Univ Concepcion, Dept Oceanog, Concepcion, Chile.
   Univ Concepcion, Ctr Invest Oceanog Pacifico Sur Oriental, Concepcion, Chile.
   CEA, Inst Genom, Genoscope, Evry, France.
   VIB, Dept Plant Syst Biol, Ghent, Belgium.
   Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9000 Ghent, Belgium.
C2 UNIV PARIS 06, FRANCE
   CNRS, FRANCE
   CNRS, FRANCE
   UNIV PARIS 06, FRANCE
   UNIV CONCEPCION, CHILE
   UNIV CONCEPCION, CHILE
   CEA, FRANCE
   VIB, BELGIUM
   UNIV GHENT, BELGIUM
SI BREST
SE CNRS
IF 3.73
TC 46
TU Centre national de la recherche scientifique
   Université Pierre et Marie Curie
UR https://archimer.ifremer.fr/doc/00129/23987/21945.pdf
LA English
DT Article
CR BIOSOPE
BO L'Atalante
AB Among small photosynthetic eukaryotes that play a key role in oceanic food webs, picoplanktonic Mamiellophyceae such as Bathycoccus, Micromonas, and Ostreococcus are particularly important in coastal regions. By using a combination of cell sorting by flow cytometry, whole genome amplification (WGA), and 454 pyrosequencing, we obtained metagenomic data for two natural picophytoplankton populations from the coastal upwelling waters off central Chile. About 60% of the reads of each sample could be mapped to the genome of Bathycoccus strain from the Mediterranean Sea (RCC1105), representing a total of 9 Mbp (sample T142) and 13 Mbp (sample T149) of non-redundant Bathycoccus genome sequences. WGA did not amplify all regions uniformly, resulting in unequal coverage along a given chromosome and between chromosomes. The identity at the DNA level between the metagenomes and the cultured genome was very high (96.3% identical bases for the three larger chromosomes over a 360 kbp alignment). At least two to three different genotypes seemed to be present in each natural sample based on read mapping to Bathycoccus RCC1105 genome.
PY 2012
PD JUL
SO Plos One
SN 1932-6203
PU Public Library Science
VL 7
IS 6
UT 000305730900092
DI 10.1371/journal.pone.0039648
ID 23987
ER

EF