FN Archimer Export Format
PT J
TI Molecular identification of differentially regulated genes in the hydrothermal-vent species Bathymodiolus thermophilus and Paralvinella pandorae in response to temperature
BT 
AF BOUTET, Isabelle
   JOLLIVET, Didier
   SHILLITO, Bruce
   MORAGA, Dario
   TANGUY, Arnaud
AS 1:1,2;2:1,2;3:3;4:4;5:1,2;
FF 1:;2:;3:;4:;5:;
C1 CNRS, Biol Stn, UMR 7144, F-29682 Roscoff, France.
   Univ Paris 06, UPMC, UMR 7144,Stn Biol Roscoff, Equipe Genet & Adaptat Milieu Extreme, F-29682 Roscoff, France.
   Univ Paris 06, UPMC, UMR 7138, F-75005 Paris, France.
   Univ Bretagne Occidentale, UMR CNRS 6539, Lab Sci Environm Marin, Inst Univ Europeen Mer, F-29280 Plouzane, France.
C2 CNRS, FRANCE
   UNIV PARIS 06, FRANCE
   UNIV PARIS 06, FRANCE
   UBO, FRANCE
SI BREST
SE IUEM
IF 3.759
TC 22
TU Centre national de la recherche scientifique
   Institut de recherche pour le développement
   Institut français de recherche pour l'exploitation de la mer
   Muséum national d'histoire naturelle
   Université Nice - Sophia-Antipolis
   Université Pierre et Marie Curie
   Université de Bretagne Occidentale
   Université des Antilles
UR https://archimer.ifremer.fr/doc/00129/24014/21970.pdf
LA English
DT Article
CR BIOSPEEDO
BO L'Atalante
DE ;oyster crassostrea gigas;heat shock protein;mid atlantic ridge;cytosolic malate dehydrogenases;extracellular hemoglobins;lipid peroxidation;northeast pacific;enzyme activities;arginine kinase;dual symbiosis
AB Background: Hydrothermal vents and cold seeps represent oases of life in the deep-sea environment, but are also characterized by challenging physical and chemical conditions. The effect of temperature fluctuations on vent organisms in their habitat has not been well explored, in particular at a molecular level, most gene expression studies being conducted on coastal marine species. In order to better understand the response of hydrothermal organisms to different temperature regimes, differentially expressed genes (obtained by a subtractive suppression hybridization approach) were identified in the mussel Bathymodiolus thermophilus and the annelid Paralvinella pandorae irlandei to characterize the physiological processes involved when animals are subjected to long term exposure (2 days) at two contrasting temperatures (10 degrees versus 20 degrees C), while maintained at in situ pressures. To avoid a potential effect of pressure, the experimental animals were initially thermally acclimated for 24 hours in a pressurized vessel. Results: For each species, we produced two subtractive cDNA libraries (forward and reverse) from sets of deep-sea mussels and annelids exposed together to a thermal challenge under pressure. RNA extracted from the gills, adductor muscle, mantle and foot tissue were used for B. thermophilus. For the annelid model, whole animals (small individuals) were used. For each of the four libraries, we sequenced 200 clones, resulting in 78 and 83 unique sequences in mussels and annelids (about 20% of the sequencing effort), respectively, with only half of them corresponding to known genes. Real-time PCR was used to validate differentially expressed genes identified in the corresponding libraries. Strong expression variations have been observed for some specific genes such as the intracellular hemoglobin, the nidogen protein, and Rab7 in P. pandorae, and the SPARC protein, cyclophilin, foot protein and adhesive plaque protein in B. thermophilus. Conclusion: Our results indicate that mussels and worms are not responding in the same way to temperature variations. While the results obtained for the mussel B. thermophilus seem to indicate a metabolic depression (strong decrease in the level of mRNA expression of numerous genes) when temperature increased, the annelid P. pandorae mainly displayed a strong regulation of the mRNA encoding subunits and linkers of respiratory pigments and some proteins involved in membrane structure. In both cases, these regulations seem to be partly due to a possible cellular oxidative stress induced by the simulated thermal environment (10 degrees C to 20 degrees C). This work will serve as a starting point for studying the transcriptomic response of hydrothermal mussels and annelids in future experiments in response to thermal stress at various conditions of duration and temperature challenge.
PY 2009
PD MAY
SO Bmc Genomics
SN 1471-2164
PU Biomed Central Ltd
VL 10
IS 222
UT 000266861200001
BP 1
EP 17
DI 10.1186/1471-2164-10-222
ID 24014
ER

EF