FN Archimer Export Format PT J TI Biophysical and biochemical properties of an unusual birnavirus pathogenic for rotifers BT AF COMPS, Michel MARI, Jocelyne POISSON, Francois BONAMI, Jean-Robert AS 1:1;2:2;3:1;4:2; FF 1:;2:;3:;4:; C1 IFREMER, EXPTL AQUACULTURE STN, GIERA, F-34250 PALAVAS LES FLOTS, FRANCE. UNIV MONTPELLIER 2, PATHOL COMPARE LAB, ERPAM, F-34060 MONTPELLIER, FRANCE. C2 IFREMER, FRANCE UNIV MONTPELLIER, FRANCE IN WOS Ifremer jusqu'en 2018 IF 3.126 TC 14 UR https://archimer.ifremer.fr/doc/00129/24032/21994.pdf LA English DT Article AB A cytoplasmic dsRNA virus, rotifer birnavirus (RBV), has recently been isolated from the rotifer Brachionus plicatilis and is associated with a high mortality rate. Histologically, the viral lesions consist of characteristic inclusions, particularly amorphous dense bodies containing occluded particles. Purified virions are about 59 nm in diameter, single-shelled and display four capsomers per edge. The purified virions have a buoyant density of 1.290 (full particles) and 1.250 (empty particles) in CsCI gradients. Four major structural polypeptides of Mrs 60K, 52K, 33K and 27K were detected by SDS-PAGE. The genome is composed of two linear segments of dsRNA with Mrs of 2.45 x 106 and 2.31 × 106; additionally, small circular ssRNA molecules were detected by electrophoresis in overloaded agarose gels, but their significance is currently unknown. Except for this last feature and the structural instability of purified virions under freeze storage, all the other biochemical and biophysical characters indicate that RBV is a member of the Birnaviridae family with, for the moment, a unique position in this group. PY 1991 PD JUL SO Journal Of General Virology SN 0022-1317 PU Soc General Microbiology VL 72 UT A1991FQ45300005 BP 1229 EP 1236 DI 10.1099/0022-1317-72-6-1229 ID 24032 ER EF