FN Archimer Export Format
PT J
TI Paralytic Toxins Accumulation and Tissue Expression of alpha-Amylase and Lipase Genes in the Pacific Oyster Crassostrea gigas Fed with the Neurotoxic Dinoflagellate Alexandrium catenella
BT 
AF ROLLAND, Jean-Luc
   PELLETIER, Kevin
   MASSERET, Estelle
   RIEUVILLENEUVE, Fabien
   SAVAR, Veronique
   SANTINI, Adrien
   AMZIL, Zouher
AS 1:1;2:1;3:1;4:1;5:2;6:1;7:2;
FF 1:PDG-RBE-BOME-LALR;2:;3:;4:;5:PDG-ODE-LER-PHYC;6:PDG-RBE-RMPF-EP;7:PDG-ODE-LER-PHYC;
C1 Univ Montpellier 2, IFREMER, Ctr Natl Rech Sci, IRD,UM1,UMR Ecol Syst Marins Cotiers 5119, F-34095 Montpellier 5, France.
   IFREMER, Dept Environm Microbiol & Phycotoxines EMP PHYC, F-44311 Nantes 3, France.
C2 UNIV MONTPELLIER, FRANCE
   IFREMER, FRANCE
SI SMEL SETE
   NANTES
   TAHITI
SE PDG-RBE-BOME-LALR
   PDG-ODE-LER-PHYC
   PDG-RBE-RMPF-EP
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.98
TC 13
UR https://archimer.ifremer.fr/doc/00130/24170/22172.pdf
LA English
DT Article
DE ;Crassostrea gigas;Alexandrium catenella;PSP toxins;digestion;gene expression
AB The pacific oyster Crassostrea gigas was experimentally exposed to the neurotoxic Alexandrium catenella and a non-producer of PSTs, Alexandrium tamarense (control algae), at concentrations corresponding to those observed during the blooming period. At fixed time intervals, from 0 to 48 h, we determined the clearance rate, the total filtered cells, the composition of the fecal ribbons, the profile of the PSP toxins and the variation of the expression of two a-amylase and triacylglecerol lipase precursor (TLP) genes through semi-quantitative RT-PCR. The results showed a significant decrease of the clearance rate of C. gigas fed with both Alexandrium species. However, from 29 to 48 h, the clearance rate and cell filtration activity increased only in oysters fed with A. tamarense. The toxin concentrations in the digestive gland rose above the sanitary threshold in less than 48 h of exposure and GTX6, a compound absent in A. catenella cells, accumulated. The a-amylase B gene expression level increased significantly in the time interval from 6 to 48 h in the digestive gland of oysters fed with A. tamarense, whereas the TLP gene transcript was significantly up-regulated in the digestive gland of oysters fed with the neurotoxic A. catenella. All together, these results suggest that the digestion capacity could be affected by PSP toxins.
PY 2012
PD NOV
SO Marine Drugs
SN 1660-3397
PU Mdpi Ag
VL 10
IS 11
UT 000311425800011
BP 2519
EP 2534
DI 10.3390/md10112519
ID 24170
ER

EF