FN Archimer Export Format PT J TI Diversity of symbioses between chemosynthetic bacteria and metazoans at the Guiness cold seep site (Gulf of Guinea, West Africa) BT AF DUPERRON, Sebastien RODRIGUES, Clara F. LEGER, Nelly SZAFRANSKI, Kamil DECKER, Carole OLU, Karine GAUDRON, Sylvie M. AS 1:1;2:1,2,3;3:1;4:1;5:4;6:4;7:1; FF 1:;2:;3:;4:;5:PDG-REM-EEP-LEP;6:PDG-REM-EEP-LEP;7:; C1 Univ Paris 06, UMR 7138, UPMC CNRS IRD MNHN, F-75005 Paris, France. Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal. Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal. Ctr Ifremer Brest, Dept Etud Ecosyst Profonds, Lab Environm Profond, F-29280 Plouzane, France. C2 UNIV PARIS 06, FRANCE UNIV AVEIRO, PORTUGAL UNIV AVEIRO, PORTUGAL IFREMER, FRANCE SI BREST SE PDG-REM-EEP-LEP IN WOS Ifremer jusqu'en 2018 DOAJ copubli-france copubli-europe copubli-univ-france IF 2.213 TC 20 UR https://archimer.ifremer.fr/doc/00133/24418/22432.pdf LA English DT Article CR WACS BO Pourquoi pas ? DE ;Acharax;Calyptogena;chemosynthesis;cold seeps;Elenaconcha;Gulf of Guinea;Lamellibrachia;symbiosis;Thyasira AB Fauna from deep-sea cold seeps worldwide is dominated by chemosymbiotic metazoans. Recently, investigation of new sites in the Gulf of Guinea yielded numerous new species for which symbiosis was strongly suspected. In this study, symbioses are characterized in five seep-specialist metazoans recently collected from the Guiness site located at ∼600 m depth. Four bivalve and one annelid species belonging to families previously documented to harbor chemosynthetic bacteria were investigated using bacterial marker gene sequencing, fluorescence in situ hybridization, and stable isotope analyses. Results support that all five species display chemosynthetic, sulfur-oxidizing γ-proteobacteria. Bacteria are abundant in the gills of bivalves, and in the trophosome of the siboglinid annelid. As observed for their relatives occurring at deeper sites, chemoautotrophy is a major source of carbon for animal nutrition. Although symbionts found in each host species are related to symbionts found in other metazoans from the same families, several incongruencies are observed among phylogenetic trees obtained from the different bacterial genes, suggesting a certain level of heterogeneity in symbiont strains present. Results provide new insights into the diversity, biogeography, and role of symbiotic bacteria in metazoans from the Gulf of Guinea, at a site located at an intermediate depth between the continental shelf and the deep sea. PY 2012 PD DEC SO Microbiologyopen SN 2045-8827 PU Wiley-blackwell VL 1 IS 4 UT 000209077800011 BP 467 EP 480 DI 10.1002/mbo3.47 ID 24418 ER EF