Complete genome sequence of acute viral necrosis virus associated with massive mortality outbreaks in the Chinese scallop, Chlamys farreri
|Author(s)||Ren Weicheng, Chen Haixia, Renault Tristan, Cai Yuyong, Bai Changming, Wang Chongming, Huang Jie|
|Affiliation(s)||Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Maricultural Organism Dis Control & Pathogen Mol, Qingdao 266071, Peoples R China.
Univ Gothenburg, Dept Rheumatol & Inflammat, S-40530 Gothenburg, Sweden.
Univ Gothenburg, Dept Biol & Environm Sci, S-40530 Gothenburg, Sweden.
Ifremer, Unite Sante Genet & Microgiol Mollusques, Lab Genet & Pathol Mollusques Marins, F-17390 La Tremblade, France.
|Source||Virology Journal (1743-422X) (Biomed Central Ltd), 2013-04 , Vol. 10 , P. -|
|WOS© Times Cited||59|
|Keyword(s)||Acute viral necrosis virus (AVNV), Herpesvirus, OsHV-1, Genome|
|Abstract||Acute viral necrosis virus (AVNV) is the causative agent of a serious disease resulting in high mortality in cultured Chinese scallops, Chlamys farreri. We have sequenced and analyzed the complete genome of AVNV. The AVNV genome is a linear, double-stranded DNA molecule of 210,993 bp with a nucleotide composition of 38.5% G + C. A total of 123 open reading frames were predicted to encode functional proteins, ranging from 41 to 1,878 amino acid residues. The DNA sequence of AVNV is 97% identical to that of ostreid herpesvirus 1 (OsHV-1), and the amino acid sequences of the encoded proteins of these two viruses are 94-100% identical. The genomic organization of AVNV is similar to that of OsHV-1, and consists of two unique regions (170.4 kb and 3.4 kb, respectively), each flanked by two inverted repeats (7.6 kb and 10.2 kb, respectively), with a third unique region (1.5 kb) situated between the two internal repeats. Our results indicate that AVNV is a variant of OsHV-1. The AVNV genome sequence provides information useful for understanding the evolution and divergence of OsHV-1 in marine molluscs.|