FN Archimer Export Format
PT J
TI Dissolved azaspiracids are absorbed and metabolized by blue mussels (Mytilus edulis)
OT Les azaspiracides dissous sont absorbés et métabolisés par les moules (Mytilus edulis)
BT 
AF JAUFFRAIS, Thierry
   KILCOYNE, Jane
   HERRENKNECHT, Christine
   TRUQUET, Philippe
   SECHET, Veronique
   MILES, Christopher O.
   HESS, Philipp
AS 1:;2:;3:;4:;5:;6:;7:;
FF 1:PDG-ODE-LITTORAL-PHYC;2:;3:;4:PDG-ODE-LITTORAL-PHYC;5:PDG-ODE-LITTORAL-PHYC;6:;7:PDG-ODE-LITTORAL-PHYC;
C1 IFREMER, Lab EMP PHYC, F-44311 Nantes, France.
   Inst Marine, Oranmore, Co Galway, Ireland.
   Univ Nantes, LUNAM, Fac Pharm, MMS EA2160, F-44035 Nantes, France.
   Norwegian Vet Inst, N-0106 Oslo, Norway.
C2 IFREMER, FRANCE
   MARINE INST GALWAY, IRELAND
   UNIV NANTES, FRANCE
   NORWEGIAN VET INST, NORWAY
SI NANTES
SE PDG-ODE-LITTORAL-PHYC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 2.581
TC 27
UR https://archimer.ifremer.fr/doc/00136/24757/22867.pdf
LA English
DT Article
DE ;Dissolved marine biotoxins;AZA;Tissue distribution;Bivalve molluscs;LC-MS/MS;Azaspiracid
AB The relationship between azaspiracid shellfish poisoning and a small dinoflagellate, Azadinium spinosum, has been shown recently. The organism produces AZA1 and -2, while AZA3 and other analogues are metabolic products formed in shellfish. We evaluated whether mussels were capable of accumulating dissolved AZA1 and -2, and compared the toxin profiles of these mussels at 24 h with profiles of those exposed to live or lysed A. spinosum. We also assessed the possibility of preparative production of AZA metabolites by exposing mussels to semi-purified AZA1. We exposed mussels to similar concentration of AZAs: dissolved AZA1+2 (crude extract) at 7.5 and 0.75 µg L-1, dissolved AZA1+2 (7.5 µg L-1) in combination with Isochrysis affinis galbana, and lysed and live A. spinosum cells at 1 × 105 and 1 × 104 cell mL-1. Subsequently, we dissected and analysed digestive glands, gills and remaining flesh. Mussels (whole flesh) accumulated AZAs above the regulatory limit except at the lower levels of dissolved AZAs. The toxin profile of the mussels varied significantly with treatment. The gills contained 42–46% and the digestive glands 23–24% of the total toxin load using dissolved AZAs, compared to 3–12% and 75–90%, respectively, in mussels exposed to live A. spinosum. Exposure of mussels to semi-purified AZA1 produced the metabolites AZA17 (16.5%) and AZA3 (1.7%) after 4 days of exposure, but the conversion efficiency was too low to justify using this procedure for preparative isolation.
PY 2013
PD APR
SO Toxicon
SN 0041-0101
PU Pergamon-elsevier Science Ltd
VL 65
UT 000317172200010
BP 81
EP 89
DI 10.1016/j.toxicon.2013.01.010
ID 24757
ER

EF