FN Archimer Export Format
PT J
TI Oyster sex determination is influenced by temperature - First clues in spat during first gonadic differentiation and gametogenesis
BT 
AF SANTERRE, Christelle
   SOURDAINE, Pascal
   MARC, Nicolas
   MINGANT, Christian
   ROBERT, Rene
   MARTINEZ, Anne-Sophie
AS 1:1,2,3;2:1,2,3;3:4;4:4;5:4;6:1,2,3;
FF 1:;2:;3:PDG-RBE-PFOM-PI;4:PDG-RBE-PFOM-PI;5:PDG-RBE-PFOM-PI;6:;
C1 Normandie Univ, Caen, France.
   UCBN, BIOMEA, F-14032 Caen, France.
   CNRS, INEE, FRE 3484, F-14032 Caen, France.
   Lab Physiol Invertebres IFREMER Brest, Stn Expt Argenton, F-29840 Argenton Landunvez, France.
C2 UNIV CAEN, FRANCE
   UNIV CAEN, FRANCE
   CNRS, FRANCE
   IFREMER, FRANCE
SI ARGENTON
SE PDG-RBE-PFOM-PI
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.371
TC 36
UR https://archimer.ifremer.fr/doc/00136/24767/22874.pdf
LA English
DT Article
DE ;Oyster spat;Temperature;Sex ratio;TSD;Sex determination pathway
AB The sex-determining system of Crassostrea gigas is still poorly known, especially regarding the potential influence of temperature. In order to address this question, mRNA expressions of actors of the molecular cascade (Cg-DMI, Cg-SoxE, Cg-beta-catenin, Cg-Foxl2/Cg-FoxI2os) and of Oyvlg, a germ cell marker, were investigated by real-time PCR in spat grown at different temperatures (18, 22, 25 and 28 degrees C). In parallel, gonadic differentiation, gametogenesis and sex ratios were assessed by histology at each of these temperatures. Whatever the temperature, Cg-DMI, Cg-SoxE, Cg-beta-catenin and Oyvlg expressions peaked at the same developmental stage, always after Cg-Foxl2/Cg-Foxl2os (around 40-44 dpf for spat grown at 18 degrees C). Temperatures increased the kinetics of first gonadic differentiation and gametogenesis. At 25 degrees C a significant switch occurred in sex ratio towards males and in the balance of expression between male and female genes, in favor of males. A slight gametogenesis disturbance was also observed. These results strengthen the hypotheses about the sex-determining time window and molecular cascade governing the development of C gigas, with notably the involvement of Cg-Foxl2/Cg-Foxl2os in the very early steps. They also suggest an influence of temperature on the oyster's sex determination which, associated to genetic control, would induce a mixed sex determination system (GSD +TSD). (C) 2013 Elsevier Inc. All rights reserved.
PY 2013
PD MAY
SO Comparative Biochemistry And Physiology A-molecular & Integrative Physiology
SN 1095-6433
PU Elsevier Science Inc
VL 165
IS 1
UT 000317638000009
BP 61
EP 69
DI 10.1016/j.cbpa.2013.02.007
ID 24767
ER

EF