Recent innovation in microbial source tracking using bacterial real-time PCR markers in shellfish

Type Article
Date 2013-03
Language English
Author(s) Mauffret Aourell1, Mieszkin Sophie1, Morizur Mael1, Alfiansah Yustian Rovi1, Lozach Solen1, Gourmelon MicheleORCID1
Affiliation(s) 1 : IFREMER, Microbiol Lab, EMP, RBE, F-29280 Plouzane, France.
Source Marine Pollution Bulletin (0025-326X) (Pergamon-elsevier Science Ltd), 2013-03 , Vol. 68 , N. 1-2 , P. 21-29
DOI 10.1016/j.marpolbul.2012.12.029
WOS© Times Cited 2
Note This work was funded by the European Regional Development Fund Interreg IVA Program as part of the collaborative project AquaManche
Keyword(s) Microbial source tracking, Shellfish, Bacteroidales, Real-time PCR, Intravalvular liquid, Digestive tissues
Abstract We assessed the capacity of real-time PCR markers to identify the origin of contamination in shellfish. Oyster, cockles or clams were either contaminated with fecal materials and host-associated markers designed from Bacteroidales or Catellicoccus marimammalium 16S RNA genes were extracted from their intravalvular liquid, digestive tissues or shellfish flesh. Extraction of bacterial DNA from the oyster intravalvular liquid with FastDNA spin kit for soil enabled the selected markers to be quantified in 100% of artificially contaminated samples, and the source of contamination to be identified in 13 out of 38 naturally contaminated batches from European Class B and Class C areas. However, this protocol did not enable the origin of the contamination to be identified in cockle or clam samples. Although results are promising for extracts from intravalvular liquid in oyster, it is unlikely that a single protocol could be the best across all bacterial markers and types of shellfish. (C) 2013 Elsevier Ltd. All rights reserved.
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