FN Archimer Export Format PT J TI Recent innovation in microbial source tracking using bacterial real-time PCR markers in shellfish BT AF MAUFFRET, Aourell MIESZKIN, Sophie MORIZUR, Mael ALFIANSAH, Yustian Rovi LOZACH, Solen GOURMELON, Michele AS 1:1;2:1;3:1;4:1;5:1;6:1; FF 1:PDG-RBE-EMP-MICLNR;2:PDG-RBE-EMP-MIC;3:;4:PDG-RBE-EMP-MICLNR;5:PDG-RBE-SG2M-LSEM;6:PDG-RBE-SG2M-LSEM; C1 IFREMER, Microbiol Lab, EMP, RBE, F-29280 Plouzane, France. C2 IFREMER, FRANCE SI BREST SE PDG-RBE-EMP-MICLNR PDG-RBE-EMP-MIC PDG-RBE-SG2M-LSEM IN WOS Ifremer jusqu'en 2018 IF 2.793 TC 6 UR https://archimer.ifremer.fr/doc/00137/24776/25188.pdf LA English DT Article DE ;Microbial source tracking;Shellfish;Bacteroidales;Real-time PCR;Intravalvular liquid;Digestive tissues AB We assessed the capacity of real-time PCR markers to identify the origin of contamination in shellfish. Oyster, cockles or clams were either contaminated with fecal materials and host-associated markers designed from Bacteroidales or Catellicoccus marimammalium 16S RNA genes were extracted from their intravalvular liquid, digestive tissues or shellfish flesh. Extraction of bacterial DNA from the oyster intravalvular liquid with FastDNA spin kit for soil enabled the selected markers to be quantified in 100% of artificially contaminated samples, and the source of contamination to be identified in 13 out of 38 naturally contaminated batches from European Class B and Class C areas. However, this protocol did not enable the origin of the contamination to be identified in cockle or clam samples. Although results are promising for extracts from intravalvular liquid in oyster, it is unlikely that a single protocol could be the best across all bacterial markers and types of shellfish. (C) 2013 Elsevier Ltd. All rights reserved. PY 2013 PD MAR SO Marine Pollution Bulletin SN 0025-326X PU Pergamon-elsevier Science Ltd VL 68 IS 1-2 UT 000317326500015 BP 21 EP 29 DI 10.1016/j.marpolbul.2012.12.029 ID 24776 ER EF