Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment
Type | Article | ||||||||
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Date | 2013-07 | ||||||||
Language | English | ||||||||
Author(s) | Morvezen Romain1, Cornette Florence2, Charrier Gregory3, Guinand Bruno4, Lapegue Sylvie2, Boudry Pierre5, Laroche Jean1 | ||||||||
Affiliation(s) | 1 : Inst Univ Europeen Mer, Lab Sci Environm Marin, LEMAR, UMR 6539, F-29280 Plouzane, France. 2 : IFREMER, Lab Genet & Pathol Mollusques Marins, F-17390 La Tremblade, France. 3 : Univ Gothenburg, Dept Biol & Environm Sci Tjarno, S-45296 Stromstad, Sweden. 4 : Univ Montpellier 2, UMR CNRS 5554, UMR 226, Inst Sci Evolut Montpellier, F-34095 Montpellier 5, France. 5 : IFREMER, Lab Sci Environm Marin, F-29280 Plouzane, France. |
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Source | Aquatic Living Resources (0990-7440) (Edp Sciences S A), 2013-07 , Vol. 26 , N. 3 , P. 207-213 | ||||||||
DOI | 10.1051/alr/2013052 | ||||||||
WOS© Times Cited | 13 | ||||||||
Keyword(s) | Microsatellites, Polymerase chain reaction, Parentage, Assignment, Bivalve mollusc | ||||||||
Abstract | We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops. | ||||||||
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