Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment
|Author(s)||Morvezen Romain1, Cornette Florence2, Charrier Gregory3, Guinand Bruno4, Lapegue Sylvie2, Boudry Pierre5, Laroche Jean1|
|Affiliation(s)||1 : Inst Univ Europeen Mer, Lab Sci Environm Marin, LEMAR, UMR 6539, F-29280 Plouzane, France.
2 : IFREMER, Lab Genet & Pathol Mollusques Marins, F-17390 La Tremblade, France.
3 : Univ Gothenburg, Dept Biol & Environm Sci Tjarno, S-45296 Stromstad, Sweden.
4 : Univ Montpellier 2, UMR CNRS 5554, UMR 226, Inst Sci Evolut Montpellier, F-34095 Montpellier 5, France.
5 : IFREMER, Lab Sci Environm Marin, F-29280 Plouzane, France.
|Source||Aquatic Living Resources (0990-7440) (Edp Sciences S A), 2013-07 , Vol. 26 , N. 3 , P. 207-213|
|WOS© Times Cited||11|
|Keyword(s)||Microsatellites, Polymerase chain reaction, Parentage, Assignment, Bivalve mollusc|
|Abstract||We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops.|