Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment

Type Article
Date 2013-07
Language English
Author(s) Morvezen Romain1, Cornette Florence2, Charrier Gregory3, Guinand Bruno4, Lapegue Sylvie2, Boudry PierreORCID5, Laroche Jean1
Affiliation(s) 1 : Inst Univ Europeen Mer, Lab Sci Environm Marin, LEMAR, UMR 6539, F-29280 Plouzane, France.
2 : IFREMER, Lab Genet & Pathol Mollusques Marins, F-17390 La Tremblade, France.
3 : Univ Gothenburg, Dept Biol & Environm Sci Tjarno, S-45296 Stromstad, Sweden.
4 : Univ Montpellier 2, UMR CNRS 5554, UMR 226, Inst Sci Evolut Montpellier, F-34095 Montpellier 5, France.
5 : IFREMER, Lab Sci Environm Marin, F-29280 Plouzane, France.
Source Aquatic Living Resources (0990-7440) (Edp Sciences S A), 2013-07 , Vol. 26 , N. 3 , P. 207-213
DOI 10.1051/alr/2013052
WOS© Times Cited 11
Keyword(s) Microsatellites, Polymerase chain reaction, Parentage, Assignment, Bivalve mollusc
Abstract We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops.
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Morvezen Romain, Cornette Florence, Charrier Gregory, Guinand Bruno, Lapegue Sylvie, Boudry Pierre, Laroche Jean (2013). Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment. Aquatic Living Resources, 26(3), 207-213. Publisher's official version : https://doi.org/10.1051/alr/2013052 , Open Access version : https://archimer.ifremer.fr/doc/00137/24822/