TY - JOUR T1 - Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment A1 - Morvezen,Romain A1 - Cornette,Florence A1 - Charrier,Gregory A1 - Guinand,Bruno A1 - Lapegue,Sylvie A1 - Boudry,Pierre A1 - Laroche,Jean AD - Inst Univ Europeen Mer, Lab Sci Environm Marin, LEMAR, UMR 6539, F-29280 Plouzane, France. AD - IFREMER, Lab Genet & Pathol Mollusques Marins, F-17390 La Tremblade, France. AD - Univ Gothenburg, Dept Biol & Environm Sci Tjarno, S-45296 Stromstad, Sweden. AD - Univ Montpellier 2, UMR CNRS 5554, UMR 226, Inst Sci Evolut Montpellier, F-34095 Montpellier 5, France. AD - IFREMER, Lab Sci Environm Marin, F-29280 Plouzane, France. UR - https://archimer.ifremer.fr/doc/00137/24822/ DO - 10.1051/alr/2013052 KW - Microsatellites KW - Polymerase chain reaction KW - Parentage KW - Assignment KW - Bivalve mollusc N2 - We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops. Y1 - 2013/07 PB - Edp Sciences S A JF - Aquatic Living Resources SN - 0990-7440 VL - 26 IS - 3 SP - 207 EP - 213 ID - 24822 ER -