Unusual Glycosaminoglycans from a Deep Sea Hydrothermal Bacterium Improve Fibrillar Collagen Structuring and Fibroblast Activities in Engineered Connective Tissues
|Author(s)||Senni Karim1, Gueniche Farida1, Changotade Sylvie2, Septier Dominique1, Sinquin Corinne3, Ratiskol Jacqueline3, Lutomski Didier2, Godeau Gaston1, Guezennec Jean3, Colliec-Jouault Sylvia3|
|Affiliation(s)||1 : Paris Descartes Univ, Sch Dent, Dept Biochem, PRES Sorbonne Paris Cite, F-92120 Montrouge, France.
2 : Univ Paris 13, CSPBAT LBPS, UFR SMBH, PRES Sorbonne Paris Cite,UMR CNRS 7244, F-93017 Bobigny, France.
3 : IFREMER, Lab Biotechnol & Marine Mol, F-44311 Nantes, France.
|Source||Marine Drugs (1660-3397) (Mdpi Ag), 2013-04 , Vol. 11 , N. 4 , P. 1351-1369|
|WOS© Times Cited||20|
|Keyword(s)||marine hydrothermal bacteria, glycosaminoglycan-mimetic, collagen, matrix metalloproteinases, dermal fibroblast, polysaccharides, tissue engineering|
|Abstract||Biopolymers produced by marine organisms can offer useful tools for regenerative medicine. Particularly, HE800 exopolysaccharide (HE800 EPS) secreted by a deep-sea hydrothermal bacterium displays an interesting glycosaminoglycan-like feature resembling hyaluronan. Previous studies demonstrated its effectiveness to enhance in vivo bone regeneration and to support osteoblastic cell metabolism in culture. Thus, in order to assess the usefulness of this high-molecular weight polymer in tissue engineering and tissue repair, in vitro reconstructed connective tissues containing HE800 EPS were performed. We showed that this polysaccharide promotes both collagen structuring and extracellular matrix settle by dermal fibroblasts. Furthermore, from the native HE800 EPS, a low-molecular weight sulfated derivative (HE800 DROS) displaying chemical analogy with heparan-sulfate, was designed. Thus, it was demonstrated that HE800 DROS mimics some properties of heparan-sulfate, such as promotion of fibroblast proliferation and inhibition of matrix metalloproteinase (MMP) secretion. Therefore, we suggest that the HE800EPS family can be considered as an innovative biotechnological source of glycosaminoglycan-like compounds useful to design biomaterials and drugs for tissue engineering and repair.|