FN Archimer Export Format
PT J
TI Unusual Glycosaminoglycans from a Deep Sea Hydrothermal Bacterium Improve Fibrillar Collagen Structuring and Fibroblast Activities in Engineered Connective Tissues
BT 
AF SENNI, Karim
   GUENICHE, Farida
   CHANGOTADE, Sylvie
   SEPTIER, Dominique
   SINQUIN, Corinne
   RATISKOL, Jacqueline
   LUTOMSKI, Didier
   GODEAU, Gaston
   GUEZENNEC, Jean
   COLLIEC-JOUAULT, Sylvia
AS 1:1;2:1;3:2;4:1;5:3;6:3;7:2;8:1;9:3;10:3;
FF 1:;2:;3:;4:;5:PDG-RBE-BRM-BMM;6:PDG-RBE-BRM-BMM;7:;8:;9:PDG-RBE-ADM1;10:PDG-RBE-BRM-BMM;
C1 Paris Descartes Univ, Sch Dent, Dept Biochem, PRES Sorbonne Paris Cite, F-92120 Montrouge, France.
   Univ Paris 13, CSPBAT LBPS, UFR SMBH, PRES Sorbonne Paris Cite,UMR CNRS 7244, F-93017 Bobigny, France.
   IFREMER, Lab Biotechnol & Marine Mol, F-44311 Nantes, France.
C2 UNIV PARIS 05, FRANCE
   UNIV PARIS 13, FRANCE
   IFREMER, FRANCE
SI BREST
   NANTES
SE PDG-RBE-BRM-BMM
   PDG-RBE-ADM1
IN WOS Ifremer jusqu'en 2018
   DOAJ
   copubli-france
   copubli-univ-france
IF 3.512
TC 20
TU Centre national de la recherche scientifique
   Université Paris 13 - Paris Nord
UR https://archimer.ifremer.fr/doc/00138/24973/23072.pdf
LA English
DT Article
CR HERO91
BO Le Nadir
DE ;marine hydrothermal bacteria;glycosaminoglycan-mimetic;collagen;matrix metalloproteinases;dermal fibroblast;polysaccharides;tissue engineering
AB Biopolymers produced by marine organisms can offer useful tools for regenerative medicine. Particularly, HE800 exopolysaccharide (HE800 EPS) secreted by a deep-sea hydrothermal bacterium displays an interesting glycosaminoglycan-like feature resembling hyaluronan. Previous studies demonstrated its effectiveness to enhance in vivo bone regeneration and to support osteoblastic cell metabolism in culture. Thus, in order to assess the usefulness of this high-molecular weight polymer in tissue engineering and tissue repair, in vitro reconstructed connective tissues containing HE800 EPS were performed. We showed that this polysaccharide promotes both collagen structuring and extracellular matrix settle by dermal fibroblasts. Furthermore, from the native HE800 EPS, a low-molecular weight sulfated derivative (HE800 DROS) displaying chemical analogy with heparan-sulfate, was designed. Thus, it was demonstrated that HE800 DROS mimics some properties of heparan-sulfate, such as promotion of fibroblast proliferation and inhibition of matrix metalloproteinase (MMP) secretion. Therefore, we suggest that the HE800EPS family can be considered as an innovative biotechnological source of glycosaminoglycan-like compounds useful to design biomaterials and drugs for tissue engineering and repair.
PY 2013
PD APR
SO Marine Drugs
SN 1660-3397
PU Mdpi Ag
VL 11
IS 4
UT 000318018700026
BP 1351
EP 1369
DI 10.3390/md11041351
ID 24973
ER

EF