FN Archimer Export Format
PT J
TI Novel inhibition of archaeal family-D DNA polymerase by uracil
BT 
AF RICHARDSON, Tomas T.
   GILROY, Louise
   ISHINO, Yoshizumi
   CONNOLLY, Bernard A.
   HENNEKE, Ghislaine
AS 1:1;2:1;3:2;4:1;5:3,4,5;
FF 1:;2:;3:;4:;5:PDG-REM-EEP-LMEE;
C1 Newcastle Univ, Inst Cell & Mol Biosci ICaMB, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England.
   Kyushu Univ, Grad Sch Bioresourse & Bioenvironm Sci, Dept Biosci & Biotechnol, Fukuoka 8128581, Japan.
   IFREMER, Ctr Brest, UMR 6197, LM2E, F-29280 Plouzane, France.
   CNRS, LM2E, UMR 6197, F-29280 Plouzane, France.
   Univ Brest, UEB, LM2E, UMR 6197, F-29280 Plouzane, France.
C2 UNIV NEWCASTLE, UK
   UNIV KYUSHU, JAPAN
   IFREMER, FRANCE
   CNRS, FRANCE
   UBO, FRANCE
SI BREST
SE PDG-REM-EEP-LMEE
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
   copubli-int-hors-europe
IF 8.808
TC 13
UR https://archimer.ifremer.fr/doc/00138/24975/23070.pdf
LA English
DT Article
AB Archaeal family-D DNA polymerase is inhibited by the presence of uracil in DNA template strands. When the enzyme encounters uracil, following three parameters change: DNA binding increases roughly 2-fold, the rate of polymerization slows by a factor of similar to 5 and 3'-5' proof-reading exonuclease activity is stimulated by a factor of similar to 2. Together these changes result in a significant decrease in polymerization activity and a reduction in net DNA synthesis. Pol D appears to interact with template strand uracil irrespective of its distance ahead of the replication fork. Polymerization does not stop at a defined location relative to uracil, rather a general decrease in DNA synthesis is observed. 'Trans' inhibition, the slowing of Pol D by uracil on a DNA strand not being replicated is also observed. It is proposed that Pol D is able to interact with uracil by looping out the single-stranded template, allowing simultaneous contact of both the base and the primer-template junction to give a polymerase-DNA complex with diminished extension ability.
PY 2013
PD APR
SO Nucleic Acids Research
SN 0305-1048
PU Oxford Univ Press
VL 41
IS 7
UT 000318167900037
BP 4207
EP 4218
DI 10.1093/nar/gkt083
ID 24975
ER

EF