Screening and selection of growth-promoting bacteria for Dunaliella cultures

Type Article
Date 2013-07
Language English
Author(s) Le Chevanton Myriam1, Garnier MatthieuORCID1, Bougaran GaelORCID1, Schreiber Nathalie1, Lukomska Ewa1, Berard Jean-BaptisteORCID1, Fouilland E.2, Bernard Olivier3, Cadoret Jean-Paul1
Affiliation(s) 1 : IFREMER, Lab Physiol & Biotechnol Algues, F-44300 Nantes, France.
2 : Univ Montpellier I, CNRS,Univ Montpellier 2, IRD,Stn Mediterraneenne Environm Littoral, IFREMER,Lab Ecol Syst Marins Cotiers,UMR 5119 ECO, F-34200 Sete, France.
3 : INRIA, F-06902 Sophia Antipolis, France.
Source Algal Research-biomass Biofuels And Bioproducts (2211-9264) (Elsevier Science Bv), 2013-07 , Vol. 2 , N. 3 , P. 212-222
DOI 10.1016/j.algal.2013.05.003
WOS© Times Cited 62
Keyword(s) Microalgae, Dunaliella, Bacteria, Interaction, Bacterial diversity
Abstract Previous studies have demonstrated that bacteria influence microalgal metabolism, suggesting that the selection and characterization of growth-promoting bacteria should offer a new strategy for improving industrial algal cultivation. In the present study, 48 cultivable bacteria were isolated from marine microalgae species and identified using 16S rRNA phylogenetic analysis. The recovered bacteria were found to be members of the α- and γ-Proteobacteria, Cytophaga–Flavobacterium–Bacteroides (CFB) and gram-positive monophyletic clusters. To address the effect of these bacteria on the growth of Dunaliella sp. individually, an experimental high-throughput tool was developed to simultaneously compare replicated associations. A two-step approach was used to monitor growth rate and biomass accumulation of Dunaliella sp. in mixed culture with bacteria, which proved the high-throughput device to be an efficient tool for the selection of growth-promoting bacteria. Depending on the bacterial strain involved, inhibitory effects were recorded for maximal microalgal growth rate, whereas inhibitory and stimulating effects were registered on microalgal biomass accumulation and nitrogen incorporation. Organic nitrogen remineralization by Alteromonas sp. SY007 and Muricauda sp. SY244 is discussed to explain the higher biomass and ammonium incorporation of Dunaliella sp. obtained under nitrogen-limited conditions. These bacteria could be considered as helpers for N accumulation in Dunaliella sp. cells.
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Le Chevanton Myriam, Garnier Matthieu, Bougaran Gael, Schreiber Nathalie, Lukomska Ewa, Berard Jean-Baptiste, Fouilland E., Bernard Olivier, Cadoret Jean-Paul (2013). Screening and selection of growth-promoting bacteria for Dunaliella cultures. Algal Research-biomass Biofuels And Bioproducts, 2(3), 212-222. Publisher's official version : https://doi.org/10.1016/j.algal.2013.05.003 , Open Access version : https://archimer.ifremer.fr/doc/00142/25334/