FN Archimer Export Format
PT J
TI Partial characterization of an exopolysaccharide secreted by a marine bacterium, Vibrio neocaledonicus sp nov., from New Caledonia
BT 
AF CHALKIADAKIS, Eletherios
   DUFOURCQ, R.
   SCHMITT, Sophie
   BRANDILY, Christophe
   KERVAREC, N.
   COATANEA, Denis
   AMIR, H.
   LOUBERSAC, Lionel
   CHANTEAU, S.
   GUEZENNEC, Jean
   DUPONT-ROUZEYROL, M.
   SIMON-COLIN, Christelle
AS 1:1,2;2:2;3:1;4:1;5:3;6:4;7:5;8:4;9:2;10:1;11:2;12:1;
FF 1:PDG-RBE-BRM-BMM;2:;3:PDG-RBE-BRM-BMM;4:PDG-REM-EEP-LEP;5:;6:PDG-RBE-LEADNC;7:;8:PDG-RBE-LEADNC;9:;10:PDG-RBE-ADM1;11:;12:PDG-RBE-BRM-BMM;
C1 IFREMER, Ctr Brest, BRM BMM, F-29280 Plouzane, France.
   Reseau Int Inst Pasteur, Inst Pasteur Nouvelle Caledonie, Lab Epidemiol Mol, Noumea, New Caledonia.
   Univ Bretagne Occidentale, Serv Commun RMN RPE, Brest 3, France.
   IFREMER, LEAD NC, Noumea, New Caledonia.
   Univ Nouvelle Caledonie, Lab Insulaire Vivant, Noumea, New Caledonia.
C2 IFREMER, FRANCE
   INST PASTEUR, FRANCE
   UBO, FRANCE
   IFREMER, FRANCE
   UNIV NOUVELLE CALEDONIE, FRANCE
SI NOUMEA
   BREST
   SAINT VINCENT
SE PDG-RBE-BRM-BMM
   PDG-REM-EEP-LEP
   PDG-RBE-LEADNC
   PDG-RBE-ADM1
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.386
TC 17
UR https://archimer.ifremer.fr/doc/00144/25501/24036.pdf
LA English
DT Article
DE ;biopolymers;biotechnology;exopolysaccharide;fermentation biotechnology
AB Aims Exopolysaccharides (EPS) are industrially valuable molecules with numerous useful properties. This study describes the techniques used for the identification of a novel Vibrio bacterium and preliminary characterization of its EPS. Methods and Results Bioprospection in marine intertidal areas of New Caledonia followed by screening for EPS producing brought to selection of the isolate NC470. Phylogenetic analysis (biochemical tests, gene sequencing and DNADNA relatedness) permitted to identify NC470 as a new member of the Vibrio genus. The EPS was produced in batch fermentation, purified using the ultrafiltration process and analysed by colorimetry, Fourier Transform Infrared spectroscopy, gas chromatography, Nuclear Magnetic Resonance and HPLC-size exclusion chromatography. This EPS exhibits a high N-acetyl-hexosamines and uronic acid content with a low amount of neutral sugar. The molecular mass was 672x103Da. These data are relevant for possible technological exploitation. Conclusions We propose the name Vibrio neocaledonicus sp. nov for this isolate NC470, producing an EPS with an unusual sugar composition. Comparison with other known polymers permitted to select applications for this polymer. Significance and Impact of the Study This study contributes to evaluate the marine biodiversity of New Caledonia. It also highlights the biotechnological potential of New Caledonia marine bacteria.
PY 2013
PD JUL
SO Journal Of Applied Microbiology
SN 1364-5072
PU Wiley-blackwell
VL 114
IS 6
UT 000319072900015
BP 1702
EP 1712
DI 10.1111/jam.12184
ID 25501
ER

EF