FN Archimer Export Format PT J TI Diversity of Vibrio spp. isolated at ambient environmental temperature in the Eastern English Channel as determined by pyrH sequencing BT AF TALL, Amadou HERVIO-HEATH, Dominique TEILLON, Anna BOISSET-HELBERT, C. DELESMONT, R. BODILIS, J. TOURON-BODILIS, A. AS 1:1;2:1;3:1;4:2;5:3;6:4;7:5; FF 1:PDG-RBE-SG2M-LSEM;2:PDG-RBE-SG2M-LSEM;3:PDG-RBE-EMP-MIC;4:;5:;6:;7:; C1 IFREMER, Ctr Brest, Dept Ressources Biol & Environm, Lab Microbiol LNR,Unite Environm Microbiol & Phyc, Plouzane, France. Univ Grenoble 1, Ctr Rech Macromol Vegetales CNRS, Grenoble, France. Eurofins IPL Environm, Gravelines, France. Univ Rouen, Lab Microbiol Signaux & Microenvironm, Rouen, France. EDF R&D, Lab Natl Hydraul & Environm, F-78401 Chatou, France. C2 IFREMER, FRANCE UNIV GRENOBLE, FRANCE EUROFINS IPL ENVIRONM, FRANCE UNIV ROUEN, FRANCE EDF R&D, FRANCE SI BREST SE PDG-RBE-SG2M-LSEM PDG-RBE-EMP-MIC IN WOS Ifremer jusqu'en 2018 copubli-france copubli-univ-france IF 2.386 TC 33 UR https://archimer.ifremer.fr/doc/00144/25502/24832.pdf LA English DT Article DE ;coastal environment;culturable Vibrio spp;diversity;pyrH and toxR genes partial sequencing AB Aims To describe the diversity of the culturable mesophilic and potentially pathogenic vibrios isolated at 22 and 37 degrees C on TCBS medium, in September 2009 from seawater and surface sediments. Methods and Results q-PCR assays previously selected for the identification of bacterial strains isolated at 37 degrees C were used in combination with the partial sequencing of two housekeeping genes, pyrH and toxR, to identify 315 strains isolated at 22 degrees C. The great majority of the 37 degrees C strains was identified by q-PCR assays, (five of the six species) with the predominance of Vibrio alginolyticus (85 center dot 9%) and V.harveyi (10 center dot 7%). The human pathogens V.parahaemolyticus and V.cholerae were rarely detected (two strains each). The 22 degrees C strains were successfully identified by the phylogeny analysis of pyrH and toxR genes, revealing 20 Vibrio species, with the predominance of the clam pathogen V.celticus (36 center dot 8%). The Splendidus and the Harveyi groups represented the main Vibrio group at 22 degrees C (80%) and 37 degrees C (99 center dot 5%), respectively. Conclusions The combination of q-PCR assays and the sequencing of pyrH and toxR genes highlighted two different Vibrio communities at 22 and 37 degrees C both dominated by pathogenic species for marine organisms. Significance and Impact of the Study The sequencing of the pyrH gene revealed to be a valuable tool to identify environmental Vibrio spp. strains isolated at 22 degrees C, as 92 center dot 3% of them were identified in this study. PY 2013 PD JUL SO Journal Of Applied Microbiology SN 1364-5072 PU Wiley-blackwell VL 114 IS 6 UT 000319072900016 BP 1713 EP 1724 DI 10.1111/jam.12181 ID 25502 ER EF