Distribution, prevalence, and genetic analysis of Panulirus argus virus 1 (PaV1) from the Caribbean Sea

Type Article
Date 2013-05
Language English
Author(s) Moss Jessica1, Behringer Donald2, 3, Shields Jeffrey D.1, Baeza Antonio4, 5, Aguilar-Perera Alfonso6, Bush Phillippe G., Dromer Clement7, Herrera-Moreno Alejandro8, Gittens Lester4, 9, Matthews Thomas R.10, McCord Michael R., Schaerer Michelle T.11, 12, Reynal LionelORCID7, Truelove Nathanial13, Butler Mark J.4
Affiliation(s) 1 : Virginia Inst Marine Sci, Dept Environm & Aquat Anim Hlth, Coll William & Mary, Gloucester Point, VA 23062 USA.
2 : Univ Florida, Sch Forest Resources & Conservat, Gainesville, FL 32653 USA.
3 : Univ Florida, Emerging Pathogens Inst, Gainesville, FL 32610 USA.
4 : Old Dominion Univ, Dept Biol Sci, Norfolk, VA 23529 USA.
5 : Smithsonian Marine Stn Ft Pierce, Ft Pierce, FL 34949 USA.
6 : Univ Autonoma Yucatan, Fac Med Vet & Zootecnia, Dep Biol Marina, Merida, Mexico.
7 : Inst Francais Rech Exploitat Mer Ifremer, Pointe Ft 97231, Martinique.
8 : Programa EcoMar Inc, Santo Domingo 1070011999, Dominican Rep.
9 : Govt Bahamas, Minist Agr & Marine Fisheries, Dept Marine Resources, Nassau 3028, Bahamas.
10 : Fish & Wildlife Res Inst, Florida Fish & Wildlife Conservat Commiss, Marathon, FL 33050 USA.
11 : US Naval Base, Naval Facil Engn Command NAVFAC, Environm Div, Guantanamo Bay, Cuba.
12 : Univ Puerto Rico, Dept Marine Sci, Mayaguez, PR 00681 USA.
13 : Univ Manchester, Fac Life Sci, Manchester M13 9PL, Lancs, England.
Source Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 2013-05 , Vol. 104 , N. 2 , P. 129-140
DOI 10.3354/dao02589
WOS© Times Cited 27
Keyword(s) Spiny lobster, Disease, Epidemiology, Connectivity
Abstract The pathogenic virus Panulirus argus virus 1 (PaV1) was first discovered in Caribbean spiny lobsters Panulirus argus from the Florida Keys (USA) in 1999 and has since been reported in Belize, Mexico, and Cuba; its distribution in the wider Caribbean is unknown. We collected tissue samples from adult spiny lobsters from 30 locations in 14 countries bordering the Caribbean Sea and used molecular diagnostics to assay for the presence of PaV1. PaV1 occurred primarily in the northern areas of the Caribbean, where its prevalence was highest. The virus was not found in lobsters from the southeastern Caribbean, and its prevalence was lowest in the southwestern Caribbean. DNA sequence analysis was performed on a fragment of the viral DNA to examine the genetic diversity of PaV1 on a Caribbean-wide scale. Sequence variation in the viral DNA fragment was high, with 61 unique alleles identified from 9 areas. The sharing of viral alleles in lobsters from distant locations supports the hypothesis of a strong genetic connectivity among lobsters within the Caribbean, and further supports the hypothesis that postlarvae infected with PaV1 may serve to disperse the virus over long distances.
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Moss Jessica, Behringer Donald, Shields Jeffrey D., Baeza Antonio, Aguilar-Perera Alfonso, Bush Phillippe G., Dromer Clement, Herrera-Moreno Alejandro, Gittens Lester, Matthews Thomas R., McCord Michael R., Schaerer Michelle T., Reynal Lionel, Truelove Nathanial, Butler Mark J. (2013). Distribution, prevalence, and genetic analysis of Panulirus argus virus 1 (PaV1) from the Caribbean Sea. Diseases Of Aquatic Organisms, 104(2), 129-140. Publisher's official version : https://doi.org/10.3354/dao02589 , Open Access version : https://archimer.ifremer.fr/doc/00144/25503/