FN Archimer Export Format
PT J
TI The ClC-3 chloride channel and osmoregulation in the European Sea Bass, Dicentrarchus labrax
BT 
AF BOSSUS, Maryline
   CHARMANTIER, Guy
   BLONDEAU-BIDET, Eva
   VALLETTA, Bianca
   BOULO, Viviane
   LORIN-NEBEL, Catherine
AS 1:1,2;2:1,2;3:1,2;4:1;5:1;6:1,2;
FF 1:;2:;3:;4:;5:PDG-RBE-LEADNC;6:;
C1 IFREMER, Equipe Adaptat Ecophysiol & Ontogenese, UMR5119, EcoSyM,CNRS,IRD,UM2,UM1, F-34095 Montpellier 05, France.
C2 IFREMER, FRANCE
   UNIV MONTPELLIER, FRANCE
   CNRS, FRANCE
SI SAINT VINCENT
SE PDG-RBE-LEADNC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 2.53
TC 15
UR https://archimer.ifremer.fr/doc/00149/26044/25185.pdf
LA English
DT Article
DE ;ClC-3 chloride channel;Na+/K+-ATPase;Dicentrarchus labrax;Osmoregulation;Osmosensing
AB Dicentrarchus labrax migrates between sea (SW), brackish and fresh water (FW) where chloride concentrations and requirements for chloride handling change: in FW, fish absorb chloride and restrict renal losses; in SW, they excrete chloride. In this study, the expression and localization of ClC-3 and Na+/K+-ATPase (NKA) were studied in fish adapted to SW, or exposed to FW from 10 min to 30 days. In gills, NKA-alpha 1 subunit expression transiently increased from 10 min and reached a stabilized intermediate expression level after 24 h in FW. ClC-3 co-localized with NKA in the basolateral membrane of mitochondria-rich cells (MRCs) at all conditions. The intensity of MRC ClC-3 immunostaining was significantly higher (by 50 %) 1 h after the transfer to FW, whereas the branchial ClC-3 protein expression was 30 % higher 7 days after the transfer as compared to SW. This is consistent with the increased number of immunopositive MRCs (immunostained for NKA and ClC-3). However, the ClC-3 mRNA expression was significantly lower in FW gills. In the kidney, after FW transfer, a transient decrease in NKA-alpha 1 subunit expression was followed by significantly higher stable levels from 24 h. The low ClC-3 protein expression detected at both salinities was not observed by immunocytochemistry in the SW kidney; ClC-3 was localized in the basal membrane of the collecting ducts and tubules 7 and 30 days after transfer to FW. Renal ClC-3 mRNA expression, however, seemed higher in SW than in FW. The potential role of this chloride channel ClC-3 in osmoregulatory and osmosensing mechanisms is discussed.
PY 2013
PD JUN
SO Journal Of Comparative Physiology B-biochemical Systemic And Environmental Physiology
SN 0174-1578
PU Springer Heidelberg
VL 183
IS 5
UT 000320733500005
BP 641
EP 662
DI 10.1007/s00360-012-0737-9
ID 26044
ER

EF