FN Archimer Export Format
PT J
TI Addressing the measurement of particulate organic and inorganic phosphorus in estuarine and coastal waters
BT 
AF LABRY, Claire
   YOUENOU, Agnes
   DELMAS, Daniel
   MICHELON, Pascale
AS 1:1;2:1;3:1;4:1;
FF 1:PDG-ODE-DYNECO-PELAGOS;2:PDG-ODE-DYNECO-PELAGOS;3:PDG-ODE-DYNECO-PELAGOS;4:PDG-ODE-DYNECO-PELAGOS;
C1 Ifremer Dyneco Pelagos, F-29280 Plouzane, France.
C2 IFREMER, FRANCE
SI BREST
SE PDG-ODE-DYNECO-PELAGOS
IN WOS Ifremer jusqu'en 2018
IF 2.115
TC 17
UR https://archimer.ifremer.fr/doc/00149/26046/26385.pdf
LA English
DT Article
DE ;Phosphorus;Speciation;Waters;Phytoplankton;HTC;CWO
AB The speciation of particulate phosphorus (TPP) into inorganic (PIP) and organic phosphorus (POP), has been scarcely investigated in suspended particulate matter (SPM), contrary to numerous sediment studies. This work evaluates the actual capacity of methods for TPP, POP and PIP determination in SPM to measure efficiently and specifically each pool. Methods were tested on a range of plankton-associated organic and inorganic P compounds, on microalgal cultures and estuarine samples. Then results are specifically discussed reviewing P speciation in phytoplankton. For TPP analysis, the method of Solorzano and Sharp (1980) is recommended compared with that of Aspila et al. (1976), originally dedicated to sediment, as there is one step less, the protocol is more explicit (use of MgSO4) and it provides the best results. The application of the original protocol of the Aspila method for TPP (no MgSO4) results in underestimated and highly variable values. For POP, the CWO (Chemical Wet Oxidation) method tested here succeeds in providing sufficient oxidation of organic P, but, inorganic polyphosphate is yet too significantly hydrolysed, and intracellular phosphate, an underestimated PIP fraction, contributes to the POP pool with this method. Finally the Aspila method for PIP. is the best compromise in estimating the contribution of PIP to TPP and POP by difference. Even if particulate polyphosphate is only partially hydrolysed (41-49%), intracellular phosphate is included and only a very low percentage of organic P, essentially nucleotidic P, is attacked. In any case, our findings show clear evidence of the necessity to fully describe the procedure when P speciation methods are used.
PY 2013
PD JUL
SO Continental Shelf Research
SN 0278-4343
PU Pergamon-elsevier Science Ltd
VL 60
UT 000321171500003
BP 28
EP 37
DI 10.1016/j.csr.2013.04.019
ID 26046
ER

EF