FN Archimer Export Format
PT J
TI Selective liquid chromatographic determination of trace domoic acid in seawater and phytoplankton: improvement using the o-phthaldialdehyde/9-fluorenylmethylchloroformate derivatization
BT 
AF DEVEZ, Aurelie
   DELMAS, Daniel
AS 1:1;2:1;
FF 1:PDG-ODE-DYNECO-PELAGOS;2:PDG-ODE-DYNECO-PELAGOS;
C1 IFREMER, Ctr Brest, Dept ODE DYNECO PELAGOS, F-29280 Plouzane, France.
C2 IFREMER, FRANCE
SI BREST
SE PDG-ODE-DYNECO-PELAGOS
IN WOS Ifremer jusqu'en 2018
IF 1.681
TC 5
UR https://archimer.ifremer.fr/doc/00151/26234/24297.pdf
   https://archimer.ifremer.fr/doc/00151/26234/24438.pdf
LA English
DT Article
CR PSEUTEC
BO Côtes De La Manche
AB Domoic acid (DA), a toxin produced worldwide by some species of the genus Pseudo-nitzschia, is responsible for contamination of marine molluscs, mammals, birds, and for human intoxication, and when detected in high levels results in closures of shellfish farms, thus causing severe economic losses to aquaculture. Studies on algal production of DA in cultures and field samples require sensitive methods capable of measuring trace concentrations of domoic acid. Measuring domoic acid concentrations at trace levels is still a significant challenge. A sensitive and reliable double derivatization using o-phthaldialdehyde-mercaptoethanol/9-fluorenylmethylchloroformate (OPA-MeSH/FMOC-Cl) followed by high-performance liquid chromatography with fluorescence detection has been adapted for the determination of trace levels of dissolved (dDA) and particulate domoic acid (pDA). The selectivity toward domoic acid is greatly improved by eliminating the reaction between FMOC-Cl and primary amines. Compared with the original Pocklington method, the sensitivity obtained by the double derivatization is largely enhanced (x6) and the reproducibility is significantly increased (<4% relative standard deviation [RSD] for nanomolar domoic acid concentrations). A better precision and an improved inter-operator reproducibility were obtained with this two-step derivatization optimized protocol. This methodology will be useful for monitoring domoic acid production both in Pseudo-nitzschia cultures and in natural environmental marine samples.
PY 2013
PD JUL
SO Limnology And Oceanography-methods
SN 1541-5856
PU Amer Soc Limnology Oceanography
VL 11
UT 000321486800004
BP 327
EP 336
DI 10.4319/lom.2013.11.327
ID 26234
ER

EF