FN Archimer Export Format
PT J
TI Archaeal and anaerobic methane oxidizer communities in the Sonora Margin cold seeps, Guaymas Basin (Gulf of California)
BT 
AF VIGNERON, Adrien
   CRUAUD, Perrine
   PIGNET, Patricia
   CAPRAIS, Jean-Claude
   CAMBON-BONAVITA, Marie-Anne
   GODFROY, Anne
   TOFFIN, Laurent
AS 1:1,2,3;2:1,2,3;3:1;4:4;5:1,2,3;6:1,2,3;7:1,2,3;
FF 1:PDG-REM-EEP-LMEE;2:PDG-REM-EEP-LMEE;3:PDG-REM-EEP-LMEE;4:PDG-REM-EEP-LEP;5:PDG-REM-EEP-LMEE;6:PDG-REM-EEP-LMEE;7:PDG-REM-EEP-LMEE;
C1 IFREMER, DEEP LM2E, Lab Microbiol Environm Extremes, UMR6197, F-29280 Plouzane, France.
   Univ Bretagne Occidentale, IUEM UMR6197, Lab Microbiol Environm Extremes, Plouzane, France.
   CNRS, Lab Microbiol Environm Extremes, UMR6197, Plouzane, France.
   IFREMER, Lab Environm Profonds, UMR6197, F-29280 Plouzane, France.
C2 IFREMER, FRANCE
   UBO, FRANCE
   CNRS, FRANCE
   IFREMER, FRANCE
SI BREST
SE PDG-REM-EEP-LMEE
   PDG-REM-EEP-LEP
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 9.267
TC 85
UR https://archimer.ifremer.fr/doc/00152/26295/24394.pdf
LA English
DT Article
CR BIG
BO L'Atalante
DE ;ANME;AOM;Archaea;FISH;Q-PCR;Sonora Margin
AB Cold seeps, located along the Sonora Margin transform fault in the Guaymas Basin, were extensively explored during the 'BIG' cruise in June 2010. They present a seafloor mosaic pattern consisting of different faunal assemblages and microbial mats. To investigate this mostly unknown cold and hydrocarbon-rich environment, geochemical and microbiological surveys of the sediments underlying two microbial mats and a surrounding macrofaunal habitat were analyzed in detail. The geochemical measurements suggest biogenic methane production and local advective sulfate-rich fluxes in the sediments. The distributions of archaeal communities, particularly those involved in the methane cycle, were investigated at different depths (surface to 18 cm below the sea floor (cmbsf)) using complementary molecular approaches, such as Automated method of Ribosomal Intergenic Spacer Analysis (ARISA), 16S rRNA libraries, fluorescence in situ hybridization and quantitative polymerase chain reaction with new specific primer sets targeting methanogenic and anaerobic methanotrophic lineages. Molecular results indicate that metabolically active archaeal communities were dominated by known clades of anaerobic methane oxidizers (archaeal anaerobic methanotroph (ANME)-1, -2 and -3), including a novel 'ANME-2c Sonora' lineage. ANME-2c were found to be dominant, metabolically active and physically associated with syntrophic Bacteria in sulfate-rich shallow sediment layers. In contrast, ANME-1 were more prevalent in the deepest sediment samples and presented a versatile behavior in terms of syntrophic association, depending on the sulfate concentration. ANME-3 were concentrated in small aggregates without bacterial partners in a restricted sediment horizon below the first centimetres. These niche specificities and syntrophic behaviors, depending on biological surface assemblages and environmental availability of electron donors, acceptors and carbon substrates, suggest that ANME could support alternative metabolic pathways than syntrophic anaerobic oxidation of methane.
PY 2013
PD AUG
SO Isme Journal
SN 1751-7362
PU Nature Publishing Group
VL 7
IS 8
UT 000322119600012
BP 1595
EP 1608
DI 10.1038/ismej.2013.18
ID 26295
ER

EF