Genetic investigations on the caffeine and chlorogenic acid relationship in an interspecific cross between Coffea liberica dewevrei and C-pseudozanguebariae

Type Article
Date 2013-08
Language English
Author(s) Ky Chin-Long1, Barre Philippe2, Noirot Michel3
Affiliation(s) 1 : Ctr IFREMER Tahiti, Taravao, Fr Polynesia.
2 : Ctr INRA Lusignan, Lusignan, France.
3 : IRD, UMR PVBMT, Ctr CIRAD Pole Protect Plantes, St Pierre 97410, Reunion.
Source Tree Genetics & Genomes (1614-2942) (Springer Heidelberg), 2013-08 , Vol. 9 , N. 4 , P. 1043-1049
DOI 10.1007/s11295-013-0616-x
WOS© Times Cited 7
Keyword(s) Coffea, Chlorogenic acids, Caffeine, QTL, Interspecific cross
Abstract The objective of the paper was to identify the number of major loci explaining caffeine content in coffee seeds. Investigations were based on previously published results: (1) Caffeine binds to chlorogenic acids in a 1:1 molecular ratio; (2) Between species, the caffeine content is correlated to the chlorogenic acid content; (3) Only a part of chlorogenic acids is bound to caffeine. Especially, the content ratio between caffeine and chlorogenic acids varied between species. For identifying the number of major loci, a quantitative trait locus (QTL) approach was carried out using an interspecific cross between two highly differentiated species-Coffea liberica dewevrei and Coffea pseudozanguebariae, the latter being a caffeine-free species. As main finding, two QTLs, i.e., RCQ1 and CQA1, were identified allowing us to explain up to 97 % of the caffeine content variance. RCQ1 explained variation of the caffeine/chlorogenic acid ratio and was genetically independent of the second QTL. The latter explained the part of the caffeine content which was dependent on the chlorogenic acid content. The findings also confirmed that only a part of chlorogenic acids were trapped by caffeine, as in wild species.
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