FN Archimer Export Format
PT J
TI Identification of the origin of faecal contamination in estuarine oysters using Bacteroidales and F-specific RNA bacteriophage markers
BT 
AF MIESZKIN, Sophie
   CAPRAIS, Marie-Paule
   LE MENNEC, Cecile
   LE GOFF, Manon
   EDGE, T. A.
   GOURMELON, Michele
AS 1:1;2:1;3:1;4:1;5:2;6:1;
FF 1:PDG-RBE-EMP-MIC;2:PDG-RBE-SG2M-LSEM;3:PDG-RBE-SG2M-LSEM;4:;5:;6:PDG-RBE-SG2M-LSEM;
C1 IFREMER, Lab Sante Environm & Microbiol, Unite SG2M, Dept RBE, F-29280 Plouzane, France.
   Environm Canada, Water Sci & Technol Directorate, Natl Water Res Inst, Burlington, ON L7R 4A6, Canada.
C2 IFREMER, FRANCE
   ENVIRONM CANADA, CANADA
   UBO, FRANCE
SI BREST
   NANTES
   UBO
SE PDG-RBE-EMP-MIC
   PDG-RBE-SG2M-LSEM
   UBOLEMAR
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
IF 2.386
TC 17
UR https://archimer.ifremer.fr/doc/00153/26426/25250.pdf
LA English
DT Article
DE ;Escherichia coli;Estuarine and river waters;F-specific RNA bacteriophages;host-associated Bacteroidales markers;microbial source tracking;shellfish
AB Aims: The aim of this study was to identify the origin of faecal pollution impacting the Elorn estuary (Brittany, France) by applying microbial source tracking (MST) markers in both oysters and estuarine waters. Methods and Results: The MST markers used were as follows: (i) human-, ruminant- and pig-associated Bacteroidales markers by real-time PCR and (ii) human genogroup II and animal genogroup I of F-specific RNA bacteriophages (FRNAPH) by culture/genotyping and by direct real-time reverse-transcriptase PCR. The higher occurrence of the human genogroup II of F-specific RNA bacteriophages using a culture/genotyping method, and human-associated Bacteroidales marker by real-time PCR, allowed the identification of human faecal contamination as the predominant source of contamination in oysters (total of 18 oyster batches tested) and waters (total of 24 water samples tested). The importance of using the intravalvular liquids instead of digestive tissues, when applying host-associated Bacteroidales markers in oysters, was also revealed. Conclusions: This study has shown that the application of a MST toolbox of diverse bacterial and viral methods can provide multiple lines of evidence to identify the predominant source of faecal contamination in shellfish from an estuarine environment. Significance and Impact of the Study: Application of this MST toolbox is a useful approach to understand the origin of faecal contamination in shellfish harvesting areas in an estuarine setting.
PY 2013
PD SEP
SO Journal Of Applied Microbiology
SN 1364-5072
PU Wiley-blackwell
VL 115
IS 3
UT 000323105500028
BP 897
EP 907
DI 10.1111/jam.12260
ID 26426
ER

EF