FN Archimer Export Format
PT J
TI Cytotoxicity, Fractionation and Dereplication of Extracts of the Dinoflagellate Vulcanodinium rugosum, a Producer of Pinnatoxin G
BT 
AF GEIGER, Marie
   DESANGLOIS, Gwenaelle
   HOGEVEEN, Kevin
   FESSARD, Valerie
   LEPRETRE, Thomas
   MONDEGUER, Florence
   GUITTON, Yann
   HERVE, Fabienne
   SECHET, Veronique
   GROVEL, Olivier
   POUCHUS, Yves-Francois
   HESS, Philipp
AS 1:1,2;2:3;3:3;4:3;5:1,2;6:1;7:1,2;8:1;9:1;10:2;11:2;12:1;
FF 1:PDG-ODE-LITTORAL-PHYC;2:;3:;4:;5:;6:PDG-ODE-LITTORAL-PHYC;7:;8:PDG-ODE-LITTORAL-PHYC;9:PDG-ODE-LITTORAL-PHYC;10:;11:;12:PDG-ODE-LITTORAL-PHYC;
C1 IFREMER, Ctr Atlantique, Lab Phycotoxines, F-44311 Nantes, France.
   Univ Nantes, LUNAM, Fac Pharm, MMS EA2160, F-44035 Nantes, France.
   ANSES, Unite Toxicol Contaminants, F-35302 Fougeres, France.
C2 IFREMER, FRANCE
   UNIV NANTES, FRANCE
   ANSES, FRANCE
SI NANTES
SE PDG-ODE-LITTORAL-PHYC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.512
TC 11
UR https://archimer.ifremer.fr/doc/00156/26723/24802.pdf
LA English
DT Article
DE ;dereplication;cyclic imine;HRMS;bioactivity;pinnatoxins
AB Pinnatoxin G (PnTX-G) is a marine toxin belonging to the class of cyclic imines and produced by the dinoflagellate Vulcanodinium rugosum. In spite of its strong toxicity to mice, leading to the classification of pinnatoxins into the class of “fast-acting toxins”, its hazard for human health has never been demonstrated. In this study, crude extracts of V. rugosum exhibited significant cytotoxicity against Neuro2A and KB cells. IC50 values of 0.38 µg mL−1 and 0.19 µg mL−1 were estimated on Neuro2A cells after only 24 h of incubation and on KB cells after 72 h of incubation, respectively. In the case of Caco-2 cells 48 h after exposure, the crude extract of V. rugosum induced cell cycle arrest accompanied by a dramatic increase in double strand DNA breaks, although only 40% cytotoxicity was observed at the highest concentration tested (5 µg mL−1). However, PnTX-G was not a potent cytotoxic compound as no reduction of the cell viability was observed on the different cell lines. Moreover, no effects on the cell cycle or DNA damage were observed following treatment of undifferentiated Caco-2 cells with PnTX-G. The crude extract of V. rugosum was thus partially purified using liquid-liquid partitioning and SPE clean-up. In vitro assays revealed strong activity of some fractions containing no PnTX-G. The crude extract and the most potent fraction were evaluated using full scan and tandem high resolution mass spectrometry. The dereplication revealed the presence of a major compound that could be putatively annotated as nakijiquinone A, N-carboxy-methyl-smenospongine or stachybotrin A, using the MarinLit™ database. Further investigations will be necessary to confirm the identity of the compounds responsible for the cytotoxicity and genotoxicity of the extracts of V. rugosum.
PY 2013
PD SEP
SO Marine Drugs
SN 1660-3397
PU Mdpi Ag
VL 11
IS 9
UT 000328621900016
BP 3350
EP 3371
DI 10.3390/md11093350
ID 26723
ER

EF