Effect of sampling location, release technique and time after activation on the movement characteristics of scallop (Pecten maximus) sperm

Type Article
Date 2013-07
Language English
Author(s) Suquet Marc1, Quere Claudie2, Mingant Christian1, Lebrun Luc1, Ratiskol Dominique1, Miner Philippe2, Cosson Jacky3
Affiliation(s) 1 : IFREMER, UMR 6539, Stn Expt Argenton, Argenton, France.
2 : IFREMER, UMR 6539, Plouzane, France.
3 : Univ South Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Vodnany 38925, Czech Republic.
Source Aquatic Living Resources (0990-7440) (Edp Sciences S A), 2013-07 , Vol. 26 , N. 3 , P. 215-220
DOI 10.1051/alr/2013048
WOS© Times Cited 8
Keyword(s) Spermatozoa, CASA, Maturation, Sperm motility, Bivalve, Pecten maximus
Abstract Sperm characteristics of scallops have not been well described in the scientific literature. The effects of sperm release technique (thermal shock versus serotonin injection), of sperm collection technique (testis sampling versus serotonin injection), of sperm sampling location along the genital tract, of in vitro sperm maturation, and of time post activation on scallop sperm characteristics were assessed in the present work. Whatever sperm release technique used, no significant differences were observed regarding the percentage of motile spermatozoa and the velocity of the average path (YAP). Compared to testicular sperm, a higher percentage of motile spermatozoa, YAP and intracellular adenosine triphosphate (ATP) content were observed for sperm shed after serotonin injection. From the distal part of testes up to the gonopore, an increase of the percentage of motile spermatozoa and YAP was assessed, suggesting a sperm 'maturation process' along the genital ducts. A higher increase in the percentage of motile sperm was recorded during a 5 mm incubation of testicular sperm in seawater containing 2 mM serotonin and seawater containing 10 mM caffein compared to seawater (control). In addition, a higher YAP was assessed, incubating testicular sperm in caffein, compared to control or serotonin. Then, the percentage of motile spermatozoa, YAP and intracellular ATP content exhibited a progressive reduction during the 10 h swimming period. Mean values of the percentage of motile spermatozoa, YAP, sperm track linearity (LIN) and intracellular ATP content recorded at the beginning of the movement period for sperm samples collected after intragonadal serotonin injection, were 82 +/- 7%, 162 +/- 15 mu m s(-1), 0.33 +/- 0.12 and 212 +/- 133 nmol x 10(-9) spermatozoa (n = 9 males), respectively. The present study confirms the existence of a sperm "maturation process" along scallop genital ducts. In addition, the cessation of scallop sperm movement can be explained by the exhaustion of ATP content at the end of the movement phase.
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Suquet Marc, Quere Claudie, Mingant Christian, Lebrun Luc, Ratiskol Dominique, Miner Philippe, Cosson Jacky (2013). Effect of sampling location, release technique and time after activation on the movement characteristics of scallop (Pecten maximus) sperm. Aquatic Living Resources, 26(3), 215-220. Publisher's official version : https://doi.org/10.1051/alr/2013048 , Open Access version : https://archimer.ifremer.fr/doc/00157/26852/