FN Archimer Export Format
PT J
TI Functional Divergence in Shrimp Anti-Lipopolysaccharide Factors (ALFs): From Recognition of Cell Wall Components to Antimicrobial Activity
BT 
AF ROSA, Rafael Diego
   VERGNES, Agnes
   DE LORGERIL, Julien
   GONCALVES, Priscila
   PERAZZOLO, Luciane Maria
   SAUNE, Laure
   ROMESTAND, Bernard
   FIEVET, Julie
   GUEGUEN, Yannick
   BACHERE, Evelyne
   DESTOUMIEUX-GARZON, Delphine
AS 1:1;2:1;3:1;4:2;5:2;6:1;7:1;8:1;9:1;10:1;11:1;
FF 1:;2:PDG-RBE-BOME-LALR;3:PDG-RBE-BOME-LALR;4:;5:;6:;7:;8:PDG-RBE-RMPF;9:PDG-RBE-RMPF;10:PDG-RBE-BOME;11:;
C1 Univ Montpellier 2, Univ Montpellier 1, Inst Francais Rech Exploitat Mer, Inst Rech Dev,CNRS,UMR5119, Montpellier, France.
   Univ Fed Santa Catarina, Dept Cell Biol Embryol & Genet, Lab Immunol Appl Aquaculture, Florianopolis, SC, Brazil.
C2 IFREMER, FRANCE
   UNIV FED SANTA CATARINA, BRAZIL
   UNIV MONTPELLIER, FRANCE
SI MONTPELLIER
   TAHITI
   PALAVAS
SE PDG-RBE-BOME-LALR
   PDG-RBE-RMPF
   PDG-RBE-BOME
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
   copubli-int-hors-europe
   copubli-sud
IF 3.534
TC 55
UR https://archimer.ifremer.fr/doc/00160/27077/25236.pdf
LA English
DT Article
AB Antilipopolysaccharide factors (ALFs) have been described as highly cationic polypeptides with a broad spectrum of potent antimicrobial activities. In addition, ALFs have been shown to recognize LPS, a major component of the Gram-negative bacteria cell wall, through conserved amino acid residues exposed in the four-stranded beta-sheet of their three dimensional structure. In penaeid shrimp, ALFs form a diverse family of antimicrobial peptides composed by three main variants, classified as ALF Groups A to C. Here, we identified a novel group of ALFs in shrimp (Group D ALFs), which corresponds to anionic polypeptides in which many residues of the LPS binding site are lacking. Both Group B (cationic) and Group D (anionic) shrimp ALFs were produced in a heterologous expression system. Group D ALFs were found to have impaired LPS-binding activities and only limited antimicrobial activity compared to Group B ALFs. Interestingly, all four ALF groups were shown to be simultaneously expressed in an individual shrimp and to follow different patterns of gene expression in response to a microbial infection. Group B was by far the more expressed of the ALF genes. From our results, nucleotide sequence variations in shrimp ALFs result in functional divergence, with significant differences in LPS-binding and antimicrobial activities. To our knowledge, this is the first functional characterization of the sequence diversity found in the ALF family.
PY 2013
PD JUN
SO Plos One
SN 1932-6203
PU Public Library Science
VL 8
IS 7
UT 000323350700064
DI 10.1371/journal.pone.0067937
ID 27077
ER

EF