FN Archimer Export Format PT J TI Comparative genomics of pathogenic lineages of Vibrio nigripulchritudo identifies virulence-associated traits BT AF GOUDENEGE, David LABREUCHE, Yannick KRIN, Evelyne ANSQUER, Dominique MANGENOT, Sophie CALTEAU, Alexandra MEDIGUE, Claudine MAZEL, Didier POLZ, Martin F. LE ROUX, Frederique AS 1:1;2:1;3:2,3;4:4;5:5;6:5,6;7:5,6;8:2,3;9:7;10:1; FF 1:PDG-RBE-PFOM;2:PDG-RBE-PFOM;3:;4:PDG-RBE-LEADNC;5:;6:;7:;8:;9:;10:PDG-RBE-PFOM; C1 Equipe Emergente Ifremer UPMC Genom Vibrio, F-29688 Roscoff, France. Inst Pasteur, Dept Genomes & Genet, Unite Plast Genome Bacterien, Paris, France. CNRS, UMR3525, Paris, France. IFREMER, Dept Lagons Ecosyst & Aquaculture Durable Nouvell, Noumea, New Caledonia. CEA, Inst Genom, Evry, France. CNRS, UMR8030, Lab Genom Comparat, Evry, France. MIT, Parsons Lab Environm Sci & Engn, Cambridge, MA 02139 USA. C2 IFREMER, FRANCE INST PASTEUR, FRANCE CNRS, FRANCE IFREMER, FRANCE CEA, FRANCE CNRS, FRANCE MIT, USA SI ROSCOFF SAINT VINCENT SE PDG-RBE-PFOM PDG-RBE-LEADNC IN WOS Ifremer jusqu'en 2018 copubli-france copubli-int-hors-europe IF 9.267 TC 24 UR https://archimer.ifremer.fr/doc/00160/27117/28369.pdf LA English DT Article DE ;horizontal gene transfer;nigritoxin;phylogeny;shrimp;virulence;vibrio AB Vibrio nigripulchritudo is an emerging pathogen of farmed shrimp in New Caledonia and other regions in the Indo-Pacific. The molecular determinants of V. nigripulchritudo pathogenicity are unknown; however, molecular epidemiological studies have suggested that pathogenicity is linked to particular lineages. Here, we performed high-throughput sequencing-based comparative genome analysis of 16 V. nigripulchritudo strains to explore the genomic diversity and evolutionary history of pathogen-containing lineages and to identify pathogen-specific genetic elements. Our phylogenetic analysis revealed three pathogen-containing V. nigripulchritudo clades, including two clades previously identified from New Caledonia and one novel clade comprising putatively pathogenic isolates from septicemic shrimp in Madagascar. The similar genetic distance between the three clades indicates that they have diverged from an ancestral population roughly at the same time and recombination analysis indicates that these genomes have, in the past, shared a common gene pool and exchanged genes. As each contemporary lineage is comprised of nearly identical strains, comparative genomics allowed differentiation of genetic elements specific to shrimp pathogenesis of varying severity. Notably, only a large plasmid present in all highly pathogenic (HP) strains encodes a toxin. Although less/non-pathogenic strains contain related plasmids, these are differentiated by a putative toxin locus. Expression of this gene by a non-pathogenic V. nigripulchritudo strain resulted in production of toxic culture supernatant, normally an exclusive feature of HP strains. Thus, this protein, here termed 'nigritoxin', is implicated to an extent that remains to be precisely determined in the toxicity of V. nigripulchritudo. PY 2013 PD OCT SO Isme Journal SN 1751-7362 PU Nature Publishing Group VL 7 IS 10 UT 000324869400010 BP 1985 EP 1996 DI 10.1038/ismej.2013.90 ID 27117 ER EF