FN Archimer Export Format
PT J
TI Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers
BT 
AF GEIGER, Marie
   GUITTON, Yann
   VANSTEELANDT, M.
   KERZAON, I.
   BLANCHET, Estelle
   DU PONT, T. Robiou
   FRISVAD, J. C.
   HESS, Philipp
   POUCHUS, Y. F.
   GROVEL, Olivier
AS 1:1,2;2:1;3:1;4:1,3;5:1;6:1;7:4;8:2;9:1;10:1;
FF 1:PDG-ODE-LITTORAL-PHYC;2:;3:;4:;5:;6:;7:;8:PDG-ODE-LITTORAL-PHYC;9:;10:;
C1 Univ Nantes, MMS, F-44035 Nantes, France.
   IFREMER, Phycotoxins Lab, Nantes, France.
   Univ Lyon 1, UCBL, CNRS, UMR 5557, F-69365 Lyon, France.
   Tech Univ Denmark, Ctr Microbial Biotechnol, DK-2800 Lyngby, Denmark.
C2 UNIV NANTES, FRANCE
   IFREMER, FRANCE
   UNIV LYON, FRANCE
   UNIV TECH DENMARK (DTU AQUA), DENMARK
SI NANTES
SE PDG-ODE-LITTORAL-PHYC
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-europe
   copubli-univ-france
IF 1.749
TC 20
UR https://archimer.ifremer.fr/doc/00160/27166/25523.pdf
LA English
DT Article
DE ;cytotoxicity;dereplication;marine fungi;mass spectrometry;Penicillium;Shellfish
AB In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish-farming areas, Penicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of the extracts was evaluated. Extracts obtained from shellfish-derived Penicillia exhibited higher cytotoxicity than the others. Ten of these strains were grown on various media including a medium based on mussel extract (Mytilus edulis), mussel flesh-based medium (MES), to study the influence of the mussel flesh on the production of cytotoxic compounds. The MES host-derived medium was created substituting the yeast extract of YES medium by an aqueous extract of mussel tissues, with other constituent identical to YES medium. When shellfish-derived strains of fungi were grown on MES medium, extracts were found to be more cytotoxic than on the YES medium for some of the strains. HPLC-UV/DAD-MS/MS dereplication of extracts from Penicillium marinum and P. restrictum strains grown on MES medium showed the enhancement of the production of some cytotoxic compounds. The mycotoxin patulin was detected in some P. antarcticum extracts, and its presence seemed to be related to their cytotoxicity. Thus, the enhancement of the toxicity of extracts obtained from shellfish-derived Penicillium strains grown on a host-derived medium, and the production of metabolites such as patulin suggests that a survey of mycotoxins in edible shellfish should be considered.
PY 2013
PD NOV
SO Letters In Applied Microbiology
SN 0266-8254
PU Wiley-blackwell
VL 57
IS 5
UT 000333021700001
BP 385
EP 392
DI 10.1111/lam.12143
ID 27166
ER

EF