FN Archimer Export Format PT J TI Marteiliosis in mussels: a rare disease? BT AF GOMBAC, Mitja KUSAR, D. OCEPEK, M. POGACNIK, M. ARZUL, Isabelle COURALEAU, Yann JENCIC, V. AS 1:1;2:2;3:2;4:1;5:3;6:3;7:4; FF 1:;2:;3:;4:;5:PDG-RBE-SG2M-LGPMM;6:PDG-RBE-AGSAE-LGP;7:; C1 Univ Ljubljana, Fac Vet, Inst Pathol Forens & Adm Vet Med, SI-1115 Ljubljana, Slovenia. Univ Ljubljana, Fac Vet, Inst Microbiol & Parasitol, SI-1115 Ljubljana, Slovenia. IFREMER, Lab Genet & Pathol, La Tremblade, France. Univ Ljubljana, Fac Vet, Inst Breeding & Hlth Care Wild Anim Fishes & Bees, SI-1115 Ljubljana, Slovenia. C2 UNIV LJUBLJANA, SLOVENIA UNIV LJUBLJANA, SLOVENIA IFREMER, FRANCE UNIV LJUBLJANA, SLOVENIA SI AUTRE LA TREMBLADE SE AUTRE PDG-RBE-SG2M-LGPMM PDG-RBE-AGSAE-LGP IN WOS Ifremer jusqu'en 2018 copubli-europe IF 2.056 TC 7 UR https://archimer.ifremer.fr/doc/00165/27640/26064.pdf LA English DT Article DE ;low prevalence;Marteilia refringens type M;Mediterranean mussels Mytilus galloprovincialis;sequencing;severe infection;Slovene Adriatic Sea AB Among 1280 cultured and wild adult Mediterranean mussels, Mytilus galloprovincialis, collected over a 1-year surveillance period from the Slovene Adriatic Sea, 0.3% were histologically positive for the presence of Marteilia spp. The infection was concentrated in winter. Employing the molecular methods of PCR, cloning, DNA restriction and sequencing, only Marteilia refringens type M was detected in all the infected mussels. Although all life-cycle stages of M. refringens severely infected digestive glands, only sporadic disruption of epithelial cells of digestive tubules and focal destruction of digestive tubules were observed in the infected mussels. This was the first detection of M. refringens in M. galloprovincialis from the Slovene Adriatic Sea with the lowest prevalence reported to date. In addition, our results highlight the need for sequencing to complement the established PCR-RFLP analysis for correct parasite typing. PY 2014 PD SEP SO Journal Of Fish Diseases SN 0140-7775 PU Wiley-blackwell VL 37 IS 9 UT 000340553800004 BP 805 EP 814 DI 10.1111/jfd.12174 ID 27640 ER EF