FN Archimer Export Format PT J TI The development of a loop-mediated isothermal amplification assay for rapid and sensitive detection of abalone herpesvirus DNA BT AF CHEN, Meilin KUO, S. T. RENAULT, Tristan CHANG, P. H. AS 1:1,3;2:2;3:4;4:1; FF 1:;2:;3:PDG-RBE-SG2M;4:; C1 Natl Taiwan Univ, Sch Vet Med, Inst Vet Med, Taipei 10764, Taiwan. Natl Inst Anim Hlth, Tansui, Taiwan. Tzu Chi Coll Technol, Hualien, Taiwan. IFREMER, Unite Sante Genet & Microbiol Mollusques, Lab Genet & Pathol Mollusques Marins, F-17390 La Tremblade, France. C2 UNIV NATL TAIWAN NTU, TAIWAN ANIM HLTH RES INST AHRI, TAIWAN UNIV TZU CHI TCUST, TAIWAN IFREMER, FRANCE SI AUTRE LA TREMBLADE SE AUTRE PDG-RBE-SG2M IN WOS Ifremer jusqu'en 2018 copubli-int-hors-europe IF 1.781 TC 8 UR https://archimer.ifremer.fr/doc/00166/27690/25882.pdf LA English DT Article DE ;Herpesvirus;Abalone;Haliotis diversicolor supertexta;Polymerase chain reaction;Loop-mediated isothermal amplification;SYBR green PCR AB A loop-mediated isothermal amplification (LAMP) assay was developed for the detection of abalone herpesvirus DNA. Two pairs of primers were designed, based on the sequence of the DNA polymerase gene of abalone herpesvirus. The reaction temperature and time were optimized to 63 °C and 60 min, respectively. LAMP amplicons were analyzed by 2% agarose gel electrophoresis or by visual inspection of a colour change emitted by fluorescent dye. The method developed was specific for the detection of abalone herpesvirus, without cross-reactions with other tested herpesviruses including ostreid herpesvirus 1 (OsHV-1), European eel herpesvirus, koi herpesvirus (KHV) and an avian herpesvirus. The LAMP assay was 100 folds more sensitive than a conventional PCR and 10 folds less sensitive than a SYBR Green PCR. These results indicate that the developed LAMP assay is a simple, rapid, sensitive, specific and reliable technique for the detection of abalone herpesvirus. PY 2014 PD FEB SO Journal Of Virological Methods SN 0166-0934 PU Elsevier Science Bv VL 197 UT 000336340300032 BP 199 EP 203 DI 10.1016/j.jviromet.2013.11.011 ID 27690 ER EF