FN Archimer Export Format
PT J
TI The effects of dietary carbohydrate sources and forms on metabolic response and intestinal microbiota in sea bass juveniles, Dicentrarchus labrax
BT 
AF GATESOUPE, Joel
   HUELVAN, Christine
   LE BAYON, Nicolas
   SEVERE, Armelle
   AASEN, Inga Marie
   DEGNES, Kristin F.
   MAZURAIS, David
   PANSERAT, Stephane
   ZAMBONINO-INFANTE, Jose-Luis
   KAUSHIK, Sadasivam
AS 1:1;2:2;3:2;4:2;5:3;6:3;7:2;8:1;9:2;10:1;
FF 1:;2:PDG-RBE-PFOM-ARN;3:PDG-RBE-PFOM-ARN;4:PDG-RBE-PFOM-ARN;5:;6:;7:PDG-RBE-PFOM-ARN;8:;9:PDG-RBE-PFOM-ARN;10:;
C1 INRA, UR 1067, Nutrition, Métabolisme, Aquaculture, 64310 Saint-Pée-sur-Nivelle, France
   Ifremer, Unité de Physiologie Fonctionnelle des Organismes Marins, LEMAR UMR 6539, Ifremer, Centre de Brest, Laboratoire PFOM/ARN, BP 70, 29280 Plouzané, France
   SINTEF Materials and Chemistry, NO-7465 Trondheim, Norway
C2 INRA, FRANCE
   IFREMER, FRANCE
   SINTEF, NORWAY
SI BREST
   ST PEE SUR NIVELLE
SE PDG-RBE-PFOM-ARN
IN WOS Ifremer jusqu'en 2018
   copubli-europe
IF 1.878
TC 41
UR https://archimer.ifremer.fr/doc/00166/27763/25944.pdf
LA English
DT Article
DE ;Digestive enzymes;glucose metabolism;intestinal microbiota;viscerosomatic index;gene expression;metabolite analyses;vegetable feedstuffs
AB The aim of this work was to investigate the catabolic process of three kinds of dietary carbohydrates in the gut of sea bass juveniles, with the possible contribution of the intestinal microbiota to the nutrition of the host, and the subsequent effects on intermediary metabolism. A first diet contained waxy maize (99% amylopectin), a highly digestible form of starch. A second diet was less quickly digestible due to its high amylose content of resistant starch. Two other diets contained fibre instead of starch, either only cellulose as control, or also other non-starch polysaccharides brought by lupin meal. The effect of the diets on the host confirmed previous results, with the stimulation of glucose storage in the liver in sea bass fed the starchy diets, which caused a significant increase in liver weight, while lupin meal caused an increase in visceral mass. Glycaemia was higher 7 ± 1 h after the last meal in the group fed resistant starch, compared to the other dietary groups, while the fast digestion of waxy maize resulted already in hypertriglyceridemia, possibly due to hepatic neolipogenesis. At the same sampling time, the activity of free amylase was reduced in the intestine of sea bass fed resistant starch, but maltase activity was stimulated in the brush border membranes of enterocytes in the same group, confirming thus the timely digestion of resistant starch. Hepatic mRNA transcripts indicated that glucose metabolism was oriented towards neoglucogenesis by the high-fibre diets, and towards glucose storage by the starchy diets, especially with waxy maize. The diet influenced both faecal and mucosal microbiota, though in a different way, likely due to the interaction with the host. Lupin meal seemed potentially interesting as a source of prebiotic polysaccharides, by modifying the balance between Vibrio spp. and Clostridium sp. Both forms of starch were also partly metabolized by microbiota, resulting in an increased concentration of acetate in the faeces.
PY 2014
PD FEB
SO Aquaculture
SN 0044-8486
PU Elsevier
VL 422-423
UT 000330221900006
BP 47
EP 53
DI 10.1016/j.aquaculture.2013.11.011
ID 27763
ER

EF