Characterization of molecular processes involved in the pearl formation in Pinctada margaritifera for a sustainable development of pearl farming industry in French Polynesia

Type Book section
Date 2013
Language English
Author(s) Gueguen YannickORCID1, Montagnani Caroline1, Joubert Caroline1, Marie Benjamin1, 3, Belliard Corinne1, Tayale Alexandre1, Fievet Julie1, Levy Peva1, Piquemal David2, Marin Frederic3, Le Moullac GillesORCID1, Ky Chin-Long1, Garen Pierre1, Lo Cedrick4, Saulnier Denis1
Affiliation(s) 1 : Ifremer, Centre du Pacifique, BP 7004, 98719 Taravao, Tahiti, Polynésie Française, France
2 : Skuldtech, Cap Delta, 1682 rue de la Valsière - 34790 Grabels, France
3 : Université de Bourgogne, UMR CNRS Biogéosciences, 21000 Dijon, France
4 : Service de la perliculture de Polynésie française, France
Book Recent Advances in Pearl Research—Proceedings of the International Symposium on Pearl Research 2011.Edited by Shugo Watabe, Kaoru Maeyama and Hiromichi Nagasawa. 2013. ISBN : 978-4-88704-158-5. pp.184-194
Keyword(s) pearl oyster, biomineralization, Pinctada margaritifera
Abstract Tahiti’s pearl farming industry plays a major socio-economic role in French Polynesia. In an increasingly competitive market where the production of high quality pearls becomes essential, research can help secure and ensure sustainable production. In that context, Ifremer, in close collaboration with the “direction des resources marines” (French Polynesian government agency) has developed research projects on the “sustainable development of pearl farming”.
This program is organized along 3 axes: (1) understanding the animal physiology and initiating a genetically selective breeding program of donor oysters; (2)
understanding pearl oyster larvae dispersal and recruitment; (3) understanding the molecular mechanisms underlying biomineralization processes during
shell and pearl formation. It is in this frame that we have developed a highthroughput Expressed Sequence Tags pyrosequencing program on the calcifying
mantle, combined with proteomic analyses of the shell and pearl. We analyzed 276738 EST sequences, leading to the constitution of a P. margaritifera mantle
transcripts catalogue of 82 sequences potentially involved in biomineralization. Our results provided direct evidence that our ESTs data set covers a large
number of the matrix proteins of P. margaritifera. In addition, our proteomic analysis enabled us to retrieve, in silico, all the sequences from P. margaritifera
involved in the biomineralization process already published on databases. Integration of these two methods allowed, for the first time, the global composition of calcifying tissue and calcified structures to be examined in tandem.
Full Text
File Pages Size Access
Author's final draft 21 2 MB Open access
11 1 MB Access on demand
Top of the page

How to cite 

Gueguen Yannick, Montagnani Caroline, Joubert Caroline, Marie Benjamin, Belliard Corinne, Tayale Alexandre, Fievet Julie, Levy Peva, Piquemal David, Marin Frederic, Le Moullac Gilles, Ky Chin-Long, Garen Pierre, Lo Cedrick, Saulnier Denis (2013). Characterization of molecular processes involved in the pearl formation in Pinctada margaritifera for a sustainable development of pearl farming industry in French Polynesia. In Recent Advances in Pearl Research—Proceedings of the International Symposium on Pearl Research 2011.Edited by Shugo Watabe, Kaoru Maeyama and Hiromichi Nagasawa. 2013. ISBN : 978-4-88704-158-5. pp.184-194 (Terrapub). https://archimer.ifremer.fr/doc/00167/27840/