FN Archimer Export Format PT CHAP TI Characterization of molecular processes involved in the pearl formation in Pinctada margaritifera for a sustainable development of pearl farming industry in French Polynesia BT Recent Advances in Pearl Research—Proceedings of the International Symposium on Pearl Research 2011.Edited by Shugo Watabe, Kaoru Maeyama and Hiromichi Nagasawa. 2013. ISBN : 978-4-88704-158-5. pp.184-194 AF GUEGUEN, Yannick MONTAGNANI, Caroline JOUBERT, Caroline MARIE, Benjamin BELLIARD, Corinne TAYALE, Alexandre FIEVET, Julie LEVY, Peva PIQUEMAL, David MARIN, Frederic LE MOULLAC, Gilles KY, Chin-Long GAREN, Pierre LO, Cedrick SAULNIER, Denis AS 1:1;2:1;3:1;4:1,3;5:1;6:1;7:1;8:1;9:2;10:3;11:1;12:1;13:1;14:4;15:1; FF 1:PDG-RBE-RMPF;2:PDG-RBE-BOME-LALR;3:PDG-DOP-DCOP-AQUAPOL-LBQP;4:;5:PDG-RBE-RMPF;6:PDG-RBE-RMPF-EP;7:PDG-RBE-RMPF;8:PDG-RBE-RMPF;9:;10:;11:PDG-RBE-RMPF;12:PDG-RBE-RMPF;13:PDG-RBE-RMPF;14:;15:PDG-RBE-RMPF; C1 Ifremer, Centre du Pacifique, BP 7004, 98719 Taravao, Tahiti, Polynésie Française, France Skuldtech, Cap Delta, 1682 rue de la Valsière - 34790 Grabels, France Université de Bourgogne, UMR CNRS Biogéosciences, 21000 Dijon, France Service de la perliculture de Polynésie française, France C2 IFREMER, FRANCE SKULDTECH, FRANCE UNIV BOURGOGNE, FRANCE SERV PERLICULTURE, FRANCE SI TAHITI MONTPELLIER SE PDG-RBE-RMPF PDG-RBE-BOME-LALR PDG-DOP-DCOP-AQUAPOL-LBQP PDG-RBE-RMPF-EP UR https://archimer.ifremer.fr/doc/00167/27840/26049.pdf LA English DT Book section DE ;pearl oyster;biomineralization;Pinctada margaritifera AB Tahiti’s pearl farming industry plays a major socio-economic role in French Polynesia. In an increasingly competitive market where the production of high quality pearls becomes essential, research can help secure and ensure sustainable production. In that context, Ifremer, in close collaboration with the “direction des resources marines” (French Polynesian government agency) has developed research projects on the “sustainable development of pearl farming”. This program is organized along 3 axes: (1) understanding the animal physiology and initiating a genetically selective breeding program of donor oysters; (2) understanding pearl oyster larvae dispersal and recruitment; (3) understanding the molecular mechanisms underlying biomineralization processes during shell and pearl formation. It is in this frame that we have developed a highthroughput Expressed Sequence Tags pyrosequencing program on the calcifying mantle, combined with proteomic analyses of the shell and pearl. We analyzed 276738 EST sequences, leading to the constitution of a P. margaritifera mantle transcripts catalogue of 82 sequences potentially involved in biomineralization. Our results provided direct evidence that our ESTs data set covers a large number of the matrix proteins of P. margaritifera. In addition, our proteomic analysis enabled us to retrieve, in silico, all the sequences from P. margaritifera involved in the biomineralization process already published on databases. Integration of these two methods allowed, for the first time, the global composition of calcifying tissue and calcified structures to be examined in tandem. PY 2013 ID 27840 ER EF