Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas

Type Article
Date 2013-10
Language English
Author(s) Guevelou Eric1, Huvet ArnaudORCID1, Galindo-Sanchez Clara E.2, Milan Massimo3, Quillien Virgile1, Daniel Jean-Yves1, Quere Claudie1, Boudry PierreORCID1, Corporeau Charlotte1
Affiliation(s) 1 : IFREMER, Lab Sci Environm Marin, UMR 6539, LEMAR, F-29280 Plouzane, France.
2 : CICESE, Dept Biotecnol Marina, Ensenada, Baja California, Mexico.
3 : Univ Padua, Dept Comparat Biomed & Food Sci, Padua, Italy.
Source Biology Of Reproduction (0006-3363) (Soc Study Reproduction), 2013-10 , Vol. 89 , N. 4 , P. 1-15
DOI 10.1095/biolreprod.113.109728
WOS© Times Cited 19
Keyword(s) AMP-activated protein kinase, Crassostrea gigas, gametogenesis, marine bivalve, reproduction
Abstract The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the first stage of gametogenesis, when mitotic activity and the differentiation of germinal cells occur, and were always more abundant in males than in females. Some targets of fatty acid and glucose metabolism appeared to be correlated with the expression of AMPK subunits at the mRNA level. We then analyzed the sex-specific AMPK activity by measuring the phosphorylation of the catalytic AMPK alpha protein and its expression at the protein level. Both the amount of AMPK alpha protein and threonine 172 phosphorylation appeared to be almost totally inhibited in mature female gonads at stage 3, at the time when accumulation of reserves in oocytes was promoted, while it remained at a high level in mature spermatozoa. Its activation might play a sex-dependent role in the management of energy during gametogenesis in oyster.
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Guevelou Eric, Huvet Arnaud, Galindo-Sanchez Clara E., Milan Massimo, Quillien Virgile, Daniel Jean-Yves, Quere Claudie, Boudry Pierre, Corporeau Charlotte (2013). Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas. Biology Of Reproduction, 89(4), 1-15. Publisher's official version : https://doi.org/10.1095/biolreprod.113.109728 , Open Access version : https://archimer.ifremer.fr/doc/00168/27911/