FN Archimer Export Format PT J TI Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas BT AF GUEVELOU, Eric HUVET, Arnaud GALINDO-SANCHEZ, Clara E. MILAN, Massimo QUILLIEN, Virgile DANIEL, Jean-Yves QUERE, Claudie BOUDRY, Pierre CORPOREAU, Charlotte AS 1:1;2:1;3:2;4:3;5:1;6:1;7:1;8:1;9:1; FF 1:PDG-RBE-PFOM-PI;2:PDG-RBE-PFOM-PI;3:;4:;5:PDG-RBE-PFOM-PI;6:PDG-RBE-PFOM-PI;7:PDG-RBE-PFOM-PI;8:PDG-RBE-PFOM;9:PDG-RBE-PFOM-PI; C1 IFREMER, Lab Sci Environm Marin, UMR 6539, LEMAR, F-29280 Plouzane, France. CICESE, Dept Biotecnol Marina, Ensenada, Baja California, Mexico. Univ Padua, Dept Comparat Biomed & Food Sci, Padua, Italy. C2 IFREMER, FRANCE CICESE, MEXICO UNIV PADUA, ITALY SI BREST SE PDG-RBE-PFOM-PI PDG-RBE-PFOM IN WOS Ifremer jusqu'en 2018 copubli-europe copubli-int-hors-europe copubli-sud IF 3.451 TC 22 UR https://archimer.ifremer.fr/doc/00168/27911/26470.pdf LA English DT Article DE ;AMP-activated protein kinase;Crassostrea gigas;gametogenesis;marine bivalve;reproduction AB The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the first stage of gametogenesis, when mitotic activity and the differentiation of germinal cells occur, and were always more abundant in males than in females. Some targets of fatty acid and glucose metabolism appeared to be correlated with the expression of AMPK subunits at the mRNA level. We then analyzed the sex-specific AMPK activity by measuring the phosphorylation of the catalytic AMPK alpha protein and its expression at the protein level. Both the amount of AMPK alpha protein and threonine 172 phosphorylation appeared to be almost totally inhibited in mature female gonads at stage 3, at the time when accumulation of reserves in oocytes was promoted, while it remained at a high level in mature spermatozoa. Its activation might play a sex-dependent role in the management of energy during gametogenesis in oyster. PY 2013 PD OCT SO Biology Of Reproduction SN 0006-3363 PU Soc Study Reproduction VL 89 IS 4 UT 000326925800019 BP 1 EP 15 DI 10.1095/biolreprod.113.109728 ID 27911 ER EF