Identification of Hypoxia-Regulated Genes in the Liver of Common Sole (Solea solea ) Fed Different Dietary Lipid Contents

Type Article
Date 2014-06
Language English
Author(s) Mazurais DavidORCID1, Ferraresso Serena2, Gatta Pier Paolo3, Desbruyeres Elisabeth1, Severe Armelle1, Corporeau Charlotte1, Claireaux Guy4, Bargelloni Luca2, Zambonino-Infante Jose-LuisORCID1
Affiliation(s) 1 : IFREMER, CS 10070, Unite Physiol Fonct Organismes Marins, UMR LEMAR 6539, F-29280 Plouzane, France.
2 : Univ Padua, Dept Comparat Biomed & Food Sci, I-35020 Legnaro, Italy.
3 : Univ Bologna, Alma Mater Studiorum Dipartimento Sci Med Vet, I-40064 Bologna, Italy.
4 : Univ Bretagne Occidentale, Inst Univ Europeen Mer, LEMAR UMR 6539, F-29280 Plouzane, France.
Source Marine Biotechnology (1436-2228) (Springer), 2014-06 , Vol. 16 , N. 3 , P. 277-288
DOI 10.1007/s10126-013-9545-9
WOS© Times Cited 11
Keyword(s) Fish, Hypoxia, Nutrition, Liver, Transcriptome, AMPK
Abstract Coastal systems could be affected by hypoxic events brought about by global change. These areas are essential nursery habitats for several fish species including the common sole (Solea solea L.). Tolerance of fish to hypoxia depends on species and also on their physiological condition and nutritional status. Indeed, high dietary lipid content has been recently shown to negatively impact the resistance of sole to a severe hypoxic challenge. In order to study the molecular mechanisms involved in the early response to hypoxic stress, the present work examined the hepatic transcriptome in common sole fed diets with low and high lipid content, exposed to severe hypoxia. The activity of AMP-activated protein kinase (AMPK) was also investigated through the quantification of threonine-172 phosphorylation in the alpha subunit. The results show that hypoxia consistently regulates several actors involved in energy metabolism pathways and particularly AMPKα, as well as some involved in cell growth and maintenance or unfolded protein response. Our findings reveal that (1) the expression of genes involved in biological processes with high energy cost or implicated in aerobic ATP synthesis was down-regulated by hypoxia, contrary to genes involved in neoglucogenesis or in angiogenesis, (2) the consumption of high lipid induced regulation of metabolic pathways going against this energy saving, and (3) this control was fine-tuned by the regulation of several transcriptomic factors. These results provide insight into the biological processes involved in the hepatic response to hypoxic stress and underline the negative impact of high lipid consumption on the tolerance of common sole to hypoxia
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Mazurais David, Ferraresso Serena, Gatta Pier Paolo, Desbruyeres Elisabeth, Severe Armelle, Corporeau Charlotte, Claireaux Guy, Bargelloni Luca, Zambonino-Infante Jose-Luis (2014). Identification of Hypoxia-Regulated Genes in the Liver of Common Sole (Solea solea ) Fed Different Dietary Lipid Contents. Marine Biotechnology, 16(3), 277-288. Publisher's official version : https://doi.org/10.1007/s10126-013-9545-9 , Open Access version : https://archimer.ifremer.fr/doc/00169/28062/