Cellular and biochemical responses of the oyster Crassostrea gigas to controlled exposures to metals and Alexandrium minutum

Type Article
Date 2014-02
Language English
Author(s) Haberkorn Hansy1, Lambert Christophe1, Le Goic Nelly1, Quere Claudie2, Bruneau Audrey1, Riso Ricardo1, Auffret Michel1, Soudant Philippe1
Affiliation(s) 1 : Univ Bretagne Occidentale, Inst Univ Europeen Mer, UMR 6539, Lab Sci Environm Marin,Technopole Brest Iroise, F-29280 Plouzane, France.
2 : IFREMER Ctr Brest, Lab Physiol Invertebres, Unite Physiol Fonct Organismes Marins, F-29280 Plouzane, France.
Source Aquatic Toxicology (0166-445X) (Elsevier Science Bv), 2014-02 , Vol. 147 , P. 158-167
DOI 10.1016/j.aquatox.2013.12.012
WOS© Times Cited 19
Keyword(s) Harmful algae, Metals, Oysters, Toxin accumulation, Physiological effects
Abstract Effects of simultaneous exposure of Pacific oyster, Crassostrea gigas, to both a harmful dinoflagellate that produces Paralytic Shellfish Toxins (PST), Alexandrium minutum, and cadmium (Cd) and copper (Cu), were assessed. Oysters were exposed to a mix of Cd-Cu with two different diets (i.e. A. minutum or Tisochrysis lutea) and compared to control oysters fed A. minutum or T. lutea, respectively, without metal addition. Metals and PST accumulations, digestive gland lipid composition, and cellular and biochemical hemolymph variables were measured after 4 days of exposure. Oysters exposed to Cd-Cu accumulated about thirty-six times less PSTs than oysters exposed to A. minutum alone. Exposure to Cd-Cu induced significant changes in neutral lipids (increase in diacylglycerol–DAG–and decrease in sterols) and phospholipids (decreases in phosphatidylcholine, phosphatidylethanolamine, cardiolipin and ceramide aminoethylphosphonate) of digestive gland suggesting that lipid metabolism disruptions and/or lipid peroxidation have occurred. Simultaneously, concentrations, percentages of dead cells and phenoloxidase activity of hemocytes increased in oysters exposed to metals while reactive oxygen species production of hemocytes decreased. Feeding on the harmful dinoflagellate A. minutum resulted in significant decreases in monoacylglycerol (MAG) and DAG and ether glycerides (EG), as well as significant increases in hemocyte concentration and phagocytic activity as compared to oysters fed T. lutea. Finally, the present study revealed that short-term, simultaneous exposure to Cd-Cu and A. minutum may induce antagonistic (i.e. hemocyte concentration and phagocytosis) or synergic (i.e. DAG content in digestive gland) effects upon cellular and tissular functions in oysters.
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Haberkorn Hansy, Lambert Christophe, Le Goic Nelly, Quere Claudie, Bruneau Audrey, Riso Ricardo, Auffret Michel, Soudant Philippe (2014). Cellular and biochemical responses of the oyster Crassostrea gigas to controlled exposures to metals and Alexandrium minutum. Aquatic Toxicology, 147, 158-167. Publisher's official version : https://doi.org/10.1016/j.aquatox.2013.12.012 , Open Access version : https://archimer.ifremer.fr/doc/00169/28071/