FN Archimer Export Format
PT J
TI Cellular and biochemical responses of the oyster Crassostrea gigas to controlled exposures to metals and Alexandrium minutum
BT 
AF HABERKORN, Hansy
   LAMBERT, Christophe
   LE GOIC, Nelly
   QUERE, Claudie
   BRUNEAU, Audrey
   RISO, Ricardo
   AUFFRET, Michel
   SOUDANT, Philippe
AS 1:1;2:1;3:1;4:2;5:1;6:1;7:1;8:1;
FF 1:;2:;3:;4:PDG-RBE-PFOM-PI;5:;6:;7:;8:;
C1 Univ Bretagne Occidentale, Inst Univ Europeen Mer, UMR 6539, Lab Sci Environm Marin,Technopole Brest Iroise, F-29280 Plouzane, France.
   IFREMER Ctr Brest, Lab Physiol Invertebres, Unite Physiol Fonct Organismes Marins, F-29280 Plouzane, France.
C2 UBO, FRANCE
   IFREMER, FRANCE
SI BREST
   UNIV-FRANCAISE
SE AUTRE
   PDG-RBE-PFOM-PI
   IUEM
   UBO
IN WOS Ifremer jusqu'en 2018
   copubli-france
   copubli-univ-france
IF 3.451
TC 21
UR https://archimer.ifremer.fr/doc/00169/28071/26286.pdf
LA English
DT Article
DE ;Harmful algae;Metals;Oysters;Toxin accumulation;Physiological effects
AB Effects of simultaneous exposure of Pacific oyster, Crassostrea gigas, to both a harmful dinoflagellate that produces Paralytic Shellfish Toxins (PST), Alexandrium minutum, and cadmium (Cd) and copper (Cu), were assessed. Oysters were exposed to a mix of Cd-Cu with two different diets (i.e. A. minutum or Tisochrysis lutea) and compared to control oysters fed A. minutum or T. lutea, respectively, without metal addition. Metals and PST accumulations, digestive gland lipid composition, and cellular and biochemical hemolymph variables were measured after 4 days of exposure. Oysters exposed to Cd-Cu accumulated about thirty-six times less PSTs than oysters exposed to A. minutum alone. Exposure to Cd-Cu induced significant changes in neutral lipids (increase in diacylglycerol–DAG–and decrease in sterols) and phospholipids (decreases in phosphatidylcholine, phosphatidylethanolamine, cardiolipin and ceramide aminoethylphosphonate) of digestive gland suggesting that lipid metabolism disruptions and/or lipid peroxidation have occurred. Simultaneously, concentrations, percentages of dead cells and phenoloxidase activity of hemocytes increased in oysters exposed to metals while reactive oxygen species production of hemocytes decreased. Feeding on the harmful dinoflagellate A. minutum resulted in significant decreases in monoacylglycerol (MAG) and DAG and ether glycerides (EG), as well as significant increases in hemocyte concentration and phagocytic activity as compared to oysters fed T. lutea. Finally, the present study revealed that short-term, simultaneous exposure to Cd-Cu and A. minutum may induce antagonistic (i.e. hemocyte concentration and phagocytosis) or synergic (i.e. DAG content in digestive gland) effects upon cellular and tissular functions in oysters.
PY 2014
PD FEB
SO Aquatic Toxicology
SN 0166-445X
PU Elsevier Science Bv
VL 147
UT 000331856600019
BP 158
EP 167
DI 10.1016/j.aquatox.2013.12.012
ID 28071
ER

EF